Anti-Vinculin/VCL Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01207
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Figure 1. Western blot analysis of Vinculin using anti- Vinculin antibody (A01207). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Vinculin antigen affinity purified polyclonal antibody (Catalog # A01207) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vinculin at approximately 124KD. The expected band size for Vinculin is at 124KD.
规格: 100μg/vial
价格: 315美元
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抗粘着斑蛋白VCL抗体(单克隆, hVIN-1)
武汉博士德生物工程有限公司
目录: MA1103
小鼠 单克隆 (hVIN-1)
反应物种: 人类, 小鼠, 大鼠, 鸡
应用: 免疫印迹, 免疫细胞化学, 免疫组化-冰冻切片

Figure 1. IHC analysis of Vinculin using anti-Vinculin antibody (MA1103). Vinculin was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Vinculin Antibody (MA1103) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 2. IHC analysis of Vinculin using anti-Vinculin antibody (MA1103). Vinculin was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Vinculin Antibody (MA1103) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 3. Western blot analysis of Vinculin using anti-Vinculin antibody (MA1103). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela, whole cell lysates, . Lane 2: human HepG2, whole cell lysates, . Lane 3: monkey COS-7 whole cell lysates, . Lane 4: human U-87MG whole cell lysates,. Lane 5: rat PC-12 whole cell lysates, . Lane 6: rat RH35 whole cell lysates, . Lane 7: mouse HEPA1-6 whole cell lysates, . Lane 8: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Vinculin antigen affinity purified monoclonal antibody (Catalog # MA1103) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Vinculin at approximately 124KD. The expected band size for Vinculin is at 124KD.
规格: 100μg/vial
价格: 315美元
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抗粘着斑蛋白/VCL抗体
武汉博士德生物工程有限公司
目录: PA1781
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化

Anti-Vinculin antibody, PA1781, Western blotting. Lane 1: Rat Heart Tissue Lysate . Lane 2: Rat Brain Tissue Lysate . Lane 3: Rat Liver Tissue Lysate . Lane 4: U87 Cell Lysate . Lane 5: SMMC Cell Lysate . Lane 6: HEPA Cell Lysate . Lane 7: HELA Cell Lysate. Lane 8: HT1080 Cell Lysate

Anti-Vinculin antibody, PA1781, IHC(P). IHC(P): Human Mammary Cancer Tissue

Anti-Vinculin antibody, PA1781, IHC(P). IHC(P): Rat Intestine Tissue
规格: 100μg/vial
价格: 315美元
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抗粘着斑蛋白VCL兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01207
domestic rabbit 单克隆 (AIG-22)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 免疫沉淀

Western blot analysis of Vinculin expression in (1) HepG2 cell lysate; (2) HeLa cell lysate (M01207). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL monoclonal antibody (Catalog # M01207) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCL
规格: 100微升
价格: 315美元
至产商
Anti-Vinculin/VCL Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01207-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
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抗粘着斑蛋白VCL抗体(生物素)
武汉博士德生物工程有限公司
目录: A01207-Biotin
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 0.1毫克
价格: 404美元
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抗磷酸化粘着斑蛋白(Y821)VCL抗体
武汉博士德生物工程有限公司
目录: A01207Y821
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 380美元
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抗粘着斑蛋白VCL抗体
武汉博士德生物工程有限公司
目录: A30448
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 323美元
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抗粘着斑蛋白VCL单克隆抗体
武汉博士德生物工程有限公司
目录: M01207-1
小鼠 单克隆 (AS1A35)
反应物种: 人类
应用: 免疫组化
规格: 100微升
价格: 380美元
至产商