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武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 趋化因子受体4 抗体
Anti-CXCR4 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00031
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (A00031). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # A00031) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 40KD. The expected band size for CXCR4 is at 40KD.
规格: 100μg/vial
价格: 315美元
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抗趋化因子受体4抗体
武汉博士德生物工程有限公司
目录: PA1237
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫组化
Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (PA1237). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, . Lane 2: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # PA1237) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 40-43KD. The expected band size for CXCR4 is at 40KD.
Figure 2. IHC analysis of CXCR4 using anti-CXCR4 antibody (PA1237). CXCR4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CXCR4 Antibody (PA1237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
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抗趋化因子受体4抗体
武汉博士德生物工程有限公司
目录: PA2081
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
Anti-CXCR4 antibody, PA2081, Western blotting. Lane 1: M231 Cell Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: JURKAT Cell Lysate.
规格: 100μg/vial
价格: 315美元
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抗趋化因子受体4单克隆抗体
武汉博士德生物工程有限公司
目录: M00031
domestic rabbit 单克隆 (CID-3)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (M00031). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # M00031) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CXCR4.
Figure 2. IHC analysis of CXCR4 using anti-CXCR4 antibody (M00031). CXCR4 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CXCR4 Antibody (M00031) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunofluorescent analysis of Jurkat cells, using CXCR4 Antibody .
规格: 100微升
价格: 315美元
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Anti-CXCR4 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00031-2
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (A00031-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates,. Lane 2: human PC-3 whole cell lysates,. Lane 3: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # A00031-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 36-40KD. The expected band size for CXCR4 is at 40KD.
规格: 100µg/vial
价格: 315美元
至产商
抗趋化因子受体4抗体
武汉博士德生物工程有限公司
目录: A00031-1
domestic rabbit 多克隆 (polyclonal)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
免疫细胞化学
,
免疫沉淀
,
流式细胞仪
规格: 0.1毫克
价格: 404美元
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Anti-CXCR4 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00031-3
domestic rabbit 多克隆 (polyclonal)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
流式细胞仪
规格: 100µg/vial
价格: 315美元
至产商
Anti-CXCR4 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00031-4
domestic rabbit 多克隆 (polyclonal)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
流式细胞仪
规格: 100µg/vial
价格: 315美元
至产商
抗趋化因子受体4(磷酸化S339)抗体
武汉博士德生物工程有限公司
目录: A00031S339
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
规格: 100微升
价格: 547美元
至产商
Anti-Phospho-Fusin (S339) CXCR4 Antibody
武汉博士德生物工程有限公司
目录: A00031S339-1
domestic rabbit 多克隆 (polyclonal)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
免疫细胞化学
规格: 100微升
价格: 380美元
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