抗趋化因子受体4抗体
武汉博士德生物工程有限公司
目录: PA1237
兔 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化

Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (PA1237). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, . Lane 2: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # PA1237) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 40-43KD. The expected band size for CXCR4 is at 40KD.

Figure 2. IHC analysis of CXCR4 using anti-CXCR4 antibody (PA1237). CXCR4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CXCR4 Antibody (PA1237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100 ug/vial
价格: 280美元
至产商
抗趋化因子受体4抗体
武汉博士德生物工程有限公司
目录: PA2081
兔 多克隆
反应物种: 人类
应用: 免疫印迹
抗趋化因子受体4单克隆抗体
武汉博士德生物工程有限公司
目录: M00031
兔 单克隆 (CID-3)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Figure 1. Western blot analysis of CXCR4 using anti-CXCR4 antibody (M00031). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # M00031) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CXCR4.

Figure 2. IHC analysis of CXCR4 using anti-CXCR4 antibody (M00031). CXCR4 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CXCR4 Antibody (M00031) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Immunofluorescent analysis of Jurkat cells, using CXCR4 Antibody .
规格: 100微克
价格: 299美元
至产商