domestic rabbit 多克隆
反应物种: 小鼠, 大鼠
应用: 免疫印迹
反应物种: 小鼠, 大鼠
应用: 免疫印迹
Western blot analysis of VEGF expression in rat kidney extract (lane 1). VEGF at 27KD was detected using rabbit anti-VEGF Antigen Affinity purified polyclonal antibody (Catalog # A00045) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化
domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类, 大鼠
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
Anti-VEGF Picoband antibody, PB9071-1.JPG. IHC(P): Human Lung Cancer Tissue
Anti-VEGF Picoband antibody, PB9071-2.JPG. IHC(P): Human Placenta Tissue
Anti-VEGF Picoband antibody, PB9071-3.jpg. All lanes: Anti-VEGF(PB9071) at 0.5ug/ml. Lane 1: Rat Thymus Tissue Lysate at 40ug. Lane 2: Rat Brain Tissue Lysate at 40ug. Predicted bind size: 27KD. Observed bind size: 27KD.
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定
反应物种: 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定
Figure 1. Western blot analysis of VEGF using anti-VEGF antibody (A00045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates,. Lane 2: mouse HEPA1-6 whole cell lysates,. Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF at approximately 27KD, 40KD. The expected band size for VEGF is at 25KD.
规格: 100µg/vial
价格: 315美元
至产商
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