domestic rabbit 单克隆 (BHD-18)
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫沉淀
反应物种: 人类, 大鼠
应用: 免疫印迹, 免疫沉淀
Western blot analysis of SK61 phosphorylation expression in HEK293 cell lysate (P01475). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KB1 monoclonal antibody (Catalog # P01475) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KB1
规格: 100 ŒºL
价格: 315美元
至产商
domestic rabbit 单克隆 (CHB-18)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
Western blot analysis of p70 S6 Kinase expression in 293T cell lysate (M01475). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KB1 monoclonal antibody (Catalog # M01475) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KB1
规格: 100 ŒºL
价格: 315美元
至产商
domestic rabbit 单克隆 (CDB-18)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫沉淀
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫沉淀
Western blot analysis of S6K1 expression in 293T cell lysate (M01475-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KB1 monoclonal antibody (Catalog # M01475-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KB1
规格: 100 ŒºL
价格: 315美元
至产商
domestic rabbit 单克隆 (18R84)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
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