抗胱氨酸-天冬氨酸蛋白酶9 CASP9兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00080-3
domestic rabbit 单克隆 (AOC-3)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

Western blot analysis of Caspase-9 in HeLa cell lysate treated with Camptothecin (M00080-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP9 monoclonal antibody (Catalog # M00080-3) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP9

Immunohistochemical analysis of paraffin-embedded human liver cancer, using Caspase-9 Antibody(M00080-3). CASP9 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP9 Antibody (M00080-3)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100微升
价格: 315美元
至产商
Anti-Caspase-9/CASP9 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00080-5
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
至产商
Anti-Caspase-9/CASP9 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00080-6
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
至产商
Anti-Caspase-9/CASP9 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00080-8
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪
规格: 100 µg/vial
价格: 315美元
至产商