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武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 Ctnnb1 抗体
Anti-beta Catenin/CTNNB1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00004
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Figure 1. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,. Lane 2: human PC-3 whole cell lysates,. Lane 3: human U-87MG whole cell lysates,. Lane 4: human Caco-2 whole cell lysates,. Lane 5: human Hela whole cell lysates,. Lane 6: human A549 whole cell lysates,. Lane 7: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95KD. The expected band size for CTNNB1 is at 85KD.
Figure 2. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates,. Lane 2: mouse heart tissue lysates,. Lane 3: mouse lung tissue lysates,. Lane 4: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95KD. The expected band size for CTNNB1 is at 85KD.
Figure 3. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100 ug/vial
价格: 315美元
至产商
抗beta连环蛋白/CTNNB1抗体
武汉博士德生物工程有限公司
目录: PA1212
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
免疫组化-石蜡切片
,
免疫组化-冰冻切片
Anti-beta Catenin antibody, PA1212, Western blotting. Lane 1: MM453 Cell Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: HELA Cell Lysate.
Anti-beta Catenin antibody, PA1212, IHC(P). IHC(P): Human Mammary Cancer Tissue
Anti-beta Catenin antibody, PA1212, IHC(F). IHC(F): Rat Intestine Tissue
规格: 100μg/vial
价格: 315美元
至产商
抗beta连环蛋白CTNNB1兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00004-1
domestic rabbit 单克隆 (EC-3)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
免疫沉淀
Western blot analysis of beta catenin expression in (1) A431 cell lysates; (2)PC-12 cell lysates (M00004-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 monoclonal antibody (Catalog # M00004-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1
IF analysis of immunocytochemical section of A431 cells using anti- beta Catenin antibody (M00004-1) . beta Catenin was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti-beta Catenin Antibody (M00004-1) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
规格: 100微升
价格: 315美元
至产商
抗beta连环蛋白/CTNNB1抗体
武汉博士德生物工程有限公司
目录: PA1212-1
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
Anti-beta Catenin antibody, PA1212-1, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Heart Tissue Lysate. Lane 3: Rat Testis Tissue Lysate. Lane 4: MCF-7 Cell Lysate. Lane 5: HELA Cell Lysate. Lane 6: M453 Cell Lysate. Lane 7: M231 Cell Lysate. Lane 8: HT1080 Cell Lysate
Anti-beta Catenin antibody, PA1212-1, IHC(P). IHC(P): Human Mammary Cancer Tissue
规格: 100μg/vial
价格: 315美元
至产商
Anti-beta Catenin CTNNB1 Antibody Picoband™ (monoclonal, 1F6)
武汉博士德生物工程有限公司
目录: M00004-2
小鼠 单克隆 (1F6)
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
Figure 1. Western blot analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human COLO-320 whole cell lysates, . Lane 3: human HepG2 whole cell lysates, . Lane 4: human MCF-7 whole cell lysates, . Lane 5: human SW620 whole cell lysates, . Lane 6: human 22RV1 whole cell lysates, . Lane 7: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-beta Catenin antigen affinity purified monoclonal antibody (Catalog # M00004-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Figure 2. IHC analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). beta Catenin was detected in paraffin-embedded section of human mammary cancer . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Figure 3. IHC analysis of beta Catenin using anti-beta Catenin antibody (M00004-2). beta Catenin was detected in paraffin-embedded section of human mammary cancer . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-beta Catenin Antibody (M00004-2) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
至产商
抗磷酸化beta连环蛋白(S33/S37)CTNNB1兔单克隆抗体
武汉博士德生物工程有限公司
目录: P00004
domestic rabbit 单克隆 (ICG-3)
反应物种:
人类
,
大鼠
应用:
免疫印迹
Western blot analysis of Phospho-beta Catenin (S33/S37) expression in (1) 293T cell lysate; (2) 293T cell lysate treated with calyculin A (P00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 monoclonal antibody (Catalog # P00004) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1
规格: 100微升
价格: 315美元
至产商
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