抗泛肽结合蛋白p62/p62抗体
武汉博士德生物工程有限公司
目录: PA1955
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Anti-SQSTM1/p62 antibody, PA1955, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: HELA Cell Lysate. Lane 3: U87 Cell Lysate. Lane 4: A549 Cell Lysate.

Anti-SQSTM1/p62 antibody, PA1955, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Anti-SQSTM1/p62 antibody, PA1955, ICC. ICC: Hela Cell
规格: 100μg/vial
价格: 315美元
至产商
Anti-SQSTM1/p62 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9444
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Figure 1. IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). SQSTM1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). SQSTM1 was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). SQSTM1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
Anti-SQSTM1/p62 Antibody Picoband™ (monoclonal, 3H11)
武汉博士德生物工程有限公司
目录: M00300-1
小鼠 单克隆 (3H11)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
至产商
抗p62/泛肽结合蛋白p62兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00300
domestic rabbit 单克隆 (EBB-19)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪

Western blot analysis of p62/SQSTM1 expression in SKBR-3 cell lysate (M00300). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQSTM1 monoclonal antibody (Catalog # M00300) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SQSTM1
规格: 100微升
价格: 315美元
至产商
抗泛肽结合蛋白p62/p62兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00300-3
domestic rabbit 单克隆 (21S51)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
规格: 100 µl/vial
价格: 315美元
至产商
抗磷酸化p62(Ser28)泛肽结合蛋白p62抗体
武汉博士德生物工程有限公司
目录: P00300-1
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹
规格: 100µl
价格: 435美元
至产商