小鼠 单克隆 (OKT4)
反应物种: 人类
应用: 流式细胞仪
反应物种: 人类
应用: 流式细胞仪
兔 单克隆 (DOE-3)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学
Western blot analysis of CD4 expression in THP-1 cell lysate (M00344). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD4 monoclonal antibody (Catalog # M00344) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD4
规格: 100 ug/vial
价格: 299美元
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小鼠 单克隆 (CA-4)
反应物种: 人类, 大鼠
应用: 免疫组化, 免疫细胞化学, 流式细胞仪
反应物种: 人类, 大鼠
应用: 免疫组化, 免疫细胞化学, 流式细胞仪
小鼠 单克隆 (OKT4)
反应物种: 人类
共轭标签: APC
应用: 流式细胞仪
反应物种: 人类
共轭标签: APC
应用: 流式细胞仪
小鼠 单克隆 (OKT4)
反应物种: 人类
共轭标签: PE
应用: 流式细胞仪
反应物种: 人类
共轭标签: PE
应用: 流式细胞仪
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