Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC02236
小鼠 单克隆 (OKT8)
反应物种: 人类, 小鼠
应用: 流式细胞仪
规格: 100微克, 500微克
价格: 114美元
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抗CD8 CD8A兔单克隆抗体
武汉博士德生物工程有限公司
目录: M02236-1
domestic rabbit 单克隆 (EAF-3)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学

Immunohistochemical analysis of paraffin-embedded human colon, using CD8 Antibody(M02236-1). CD8A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD8A Antibody (M02236-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of CD8 expression in Jurkat cell lysate (M02236-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD8A monoclonal antibody (Catalog # M02236-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD8A
规格: 100微升
价格: 315美元
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Anti-human CD8 Cd8a Monoclonal Antibody FITC Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC02236-FITC
小鼠 单克隆 (OKT8)
反应物种: 人类, 小鼠
共轭标签: 异硫氰酸荧光素
应用: 流式细胞仪
规格: 100试验, 200试验
价格: 132美元
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抗CD8甲CD8A抗体
武汉博士德生物工程有限公司
目录: A02236
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
规格: 100微升
价格: 697美元
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