首页
>
武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 CD163 抗体
抗CD163抗体
武汉博士德生物工程有限公司
目录: PA1874
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
Anti-CD163 antibody, PA1874, Western blotting. Recombinant Protein Detection Source: E.coli derived -recombinant human CD163, 35.2KD (162aa tag+E1002-L1156). Lane 1: Recombinant Human CD163 Protein 10ng . Lane 2: Recombinant Human CD163 Protein 5ng . Lane 3: Recombinant Human CD163 Protein 2.5ng
规格: 100μg/vial
价格: 315美元
至产商
Anti-CD163 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00812-1
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化-石蜡切片
Figure 1. Western blot analysis of CD163 using anti-CD163 antibody (A00812-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human placenta tissue lysates,. Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody (Catalog # A00812-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD163 at approximately 150KD. The expected band size for CD163 is at 128KD.
Figure 2. IHC analysis of CD163 using anti-CD163 antibody (A00812-1). CD163 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of CD163 using anti-CD163 antibody (A00812-1). CD163 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CD163 Antibody (A00812-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
至产商
抗CD163抗体
武汉博士德生物工程有限公司
目录: PA1874-1
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫组化
Anti-CD163 antibody, PA1874-1, IHC(P). IHC(P): Human Mammary Cancer Tissue
Anti-CD163 antibody, PA1874-1, IHC(P). IHC(P): Human Intestinal Cancer Tissue
Anti-CD163 antibody, PA1874-1, Western blotting. All lanes: Anti CD163 (PA1874-1) at 0.5ug/ml. Lane 1: Recombinant Human CD163 Protein 10ng. Lane 2: Recombinant Human CD163 Protein 5ng. Lane 3: Recombinant Human CD163 Protein 2.5ng. Predicted bind size: 35KD. Observed bind size: 35KD
规格: 100μg/vial
价格: 315美元
至产商
抗人类CD163 DyLight系列染料®488共轭标签抗体
武汉博士德生物工程有限公司
目录: A00812-Dyl488
domestic rabbit 多克隆
反应物种:
人类
共轭标签: DyLight488
应用:
流式细胞仪
Boster Kit Box
规格: 50 ug/vial
价格: 415美元
至产商
抗人类CD163 DyLight系列染料®550共轭标签抗体
武汉博士德生物工程有限公司
目录: A00812-Dyl550
domestic rabbit 多克隆
反应物种:
人类
共轭标签: DyLight系列染料550
应用:
流式细胞仪
Boster Kit Box
规格: 50 ug/vial
价格: 415美元
至产商
Anti-CD163 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00812-3
domestic rabbit 多克隆 (polyclonal)
反应物种:
人类
应用:
免疫印迹
,
流式细胞仪
规格: 100 µg/vial
价格: 315美元
至产商
抗CD163兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00812
domestic rabbit 单克隆 (ABCC-3)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫沉淀
规格: 100微升
价格: 315美元
至产商
抗CD163兔单克隆抗体, Clone#RM371
武汉博士德生物工程有限公司
目录: M00812-1
domestic rabbit 单克隆 (RM371)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
规格: 100微升
价格: 399美元
至产商
微博分享
关注我们的微博
来邦网
英文来邦