抗CD44兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00052-1
domestic rabbit 单克隆 (AGI-3)
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Western blot analysis of CD44 expression in TF-1 cells lysate (M00052-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 monoclonal antibody (Catalog # M00052-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44

Immunohistochemical analysis of paraffin-embedded human skin, using CD44 Antibody(M00052-1). CD44 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD44 Antibody (M00052-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100微升
价格: 315美元
至产商
抗CD44抗原CD44抗体
武汉博士德生物工程有限公司
目录: PA1021-2
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学
Anti-CD44 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00052
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

Figure 1. Western blot analysis of CD44 using anti-CD44 antibody (A00052). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate,. Lane 2: human 22RV1 cell lysate,. Lane 3: rat spleen tissue lysate,. Lane 4: rat thymus tissue lysate,. Lane 5: mouse spleen tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody (Catalog # A00052) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44 at approximately 88KD. The expected band size for CD44 is at 82KD.

Figure 2. IHC analysis of CD44 using anti-CD44 antibody (A00052). CD44 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD44 Antibody (A00052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of CD44 using anti-CD44 antibody (A00052). CD44 was detected in paraffin-embedded section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD44 Antibody (A00052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
至产商
抗磷酸化CD44(S706)抗体
武汉博士德生物工程有限公司
目录: A00052S706
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格: 100微升
价格: 380美元
至产商
Anti-Human CD44 Monoclonal Antibody Unconjugated Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC00052
小鼠 单克隆 (Hermes-3)
反应物种: 人类
应用: 流式细胞仪
规格: 100微克, 500微克
价格: 147美元
至产商
Anti-Human CD44 Monoclonal Antibody PE Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC00052-PE
小鼠 单克隆 (Hermes-3)
反应物种: 人类
共轭标签: PE
应用: 流式细胞仪
规格: 100试验, 200试验
价格: 279美元
至产商
抗CD44兔单克隆抗体, Clone#RM264
武汉博士德生物工程有限公司
目录: M00052-2
domestic rabbit 单克隆 (RM264)
反应物种: 人类
应用: 免疫印迹, 免疫组化
规格: 100微升
价格: 399美元
至产商
Anti-CD44 Picoband™ Antibody (monoclonal, 7H7)
武汉博士德生物工程有限公司
目录: M00052-3
小鼠 单克隆 (7H7)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪
规格: 100µg/vial
价格: 315美元
至产商