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武汉博士德生物工程有限公司
> 武汉博士德生物工程有限公司 CD44 抗体
Anti-CD44 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9333
domestic rabbit 多克隆
反应物种:
人类
,
大鼠
应用:
免疫印迹
,
免疫细胞化学
,
免疫组化-石蜡切片
Anti- CD44 Picoband antibody, PB9333, Western blotting. All lanes: Anti CD44 (PB9333) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: PANC Whole Cell Lysate at 40ug. Predicted bind size: 82KD. Observed bind size: 115KD
Anti- CD44 Picoband antibody, PB9333, IHC(P). IHC(P): Human Intestinal Cancer Tissue
Figure 3. IF analysis of CD44 using anti-CD44 antibody (PB9333) . CD44 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
规格: 100μg/vial
价格: 315美元
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抗CD44兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00052-1
domestic rabbit 单克隆 (AGI-3)
反应物种:
人类
,
小鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
流式细胞仪
Western blot analysis of CD44 expression in TF-1 cells lysate (M00052-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 monoclonal antibody (Catalog # M00052-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44
Immunohistochemical analysis of paraffin-embedded human skin, using CD44 Antibody(M00052-1). CD44 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD44 Antibody (M00052-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100微升
价格: 315美元
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抗CD44抗原CD44抗体
武汉博士德生物工程有限公司
目录: PA1021-2
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
Anti-CD44 antibody, PA1021-2, IHC(P). IHC(P): Human Intestinal Cancer Tissue
Anti-CD44 antibody, PA1021-2, IHC(P). IHC(P): Rat Intestine Tissue
Anti-CD44 antibody, PA1021-2, ICC. ICC: HCT116 Cell
规格: 100μg/vial
价格: 315美元
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Anti-Human CD44 Monoclonal Antibody FITC Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC00052-FITC
小鼠 单克隆 (Hermes-3)
反应物种:
人类
共轭标签: 异硫氰酸荧光素
应用:
流式细胞仪
Lymphocytes gated PBMCs stained with FITC conjugated anti-human CD44 (clone Hermes-3) (red histogram). Lymphocytes gated PBMCs stained with FITC conjugated mouse IgG2a isotype control (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by using FlowJo software.
规格: 100试验, 200试验
价格: 235美元
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Anti-CD44 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A00052
domestic rabbit 多克隆
反应物种:
人类
,
小鼠
,
大鼠
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫细胞化学
,
流式细胞仪
,
免疫组化-石蜡切片
,
免疫组化-冰冻切片
Figure 1. Western blot analysis of CD44 using anti-CD44 antibody (A00052). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate,. Lane 2: human 22RV1 cell lysate,. Lane 3: rat spleen tissue lysate,. Lane 4: rat thymus tissue lysate,. Lane 5: mouse spleen tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody (Catalog # A00052) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44 at approximately 88KD. The expected band size for CD44 is at 82KD.
Figure 2. IHC analysis of CD44 using anti-CD44 antibody (A00052). CD44 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD44 Antibody (A00052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of CD44 using anti-CD44 antibody (A00052). CD44 was detected in paraffin-embedded section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD44 Antibody (A00052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
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抗磷酸化CD44(S706)抗体
武汉博士德生物工程有限公司
目录: A00052S706
domestic rabbit 多克隆 (polyclonal)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
规格: 100微升
价格: 380美元
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Anti-Human CD44 Monoclonal Antibody Unconjugated Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC00052
小鼠 单克隆 (Hermes-3)
反应物种:
人类
应用:
流式细胞仪
规格: 100微克, 500微克
价格: 147美元
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Anti-Human CD44 Monoclonal Antibody PE Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC00052-PE
小鼠 单克隆 (Hermes-3)
反应物种:
人类
共轭标签: PE
应用:
流式细胞仪
规格: 100试验, 200试验
价格: 279美元
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抗CD44兔单克隆抗体, Clone#RM264
武汉博士德生物工程有限公司
目录: M00052-2
domestic rabbit 单克隆 (RM264)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
规格: 100微升
价格: 399美元
至产商
Anti-CD44 Picoband™ Antibody (monoclonal, 7H7)
武汉博士德生物工程有限公司
目录: M00052-3
小鼠 单克隆 (7H7)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
流式细胞仪
规格: 100µg/vial
价格: 315美元
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