抗CD81兔单克隆抗体
武汉博士德生物工程有限公司
目录: M01281-1
domestic rabbit 单克隆 (GAA-3)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Western blot analysis of CD81 expression in Ramos cell lysate (M01281-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD81 monoclonal antibody (Catalog # M01281-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD81

IHC analysis of paraffin-embedded section of human tonsil tissues using anti-CD81 antibody (M01281-1). CD81 was detected in paraffin-embedded. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD81 Antibody (M01281-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100微升
价格: 315美元
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Anti-TAPA1/CD81 Antibody Picoband™
武汉博士德生物工程有限公司
目录: A01281-2
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

Figure 1. IHC analysis of TAPA1 using anti-TAPA1 antibody (A01281-2). TAPA1 was detected in paraffin-embedded section of human tonsil tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAPA1 Antibody (A01281-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. Flow Cytometry analysis of PBMC cells using anti-TAPA1 antibody (A01281-2). Overlay histogram showing PBMC cells stained with A01281-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (A01281-2,1ug/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Figure 3. Flow Cytometry analysis of Jurkat cells using anti-TAPA1 antibody (A01281-2). Overlay histogram showing Jurkat cells stained with A01281-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (A01281-2,1ug/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
规格: 100µg/vial
价格: 315美元
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抗人类TAPA1 DyLight系列染料®488共轭标签CD81抗体
武汉博士德生物工程有限公司
目录: A01281-Dyl488
domestic rabbit 多克隆
反应物种: 人类
共轭标签: DyLight488
应用: 流式细胞仪

Boster Kit Box
规格: 50 ug/vial
价格: 415美元
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抗人类TAPA1 DyLight系列染料®550共轭标签CD81抗体
武汉博士德生物工程有限公司
目录: A01281-Dyl550
domestic rabbit 多克隆
反应物种: 人类
共轭标签: DyLight系列染料550
应用: 流式细胞仪

Boster Kit Box
规格: 50 ug/vial
价格: 415美元
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抗CD81抗原CD81抗体
武汉博士德生物工程有限公司
目录: A01281
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
规格: 0.1毫克
价格: 404美元
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Anti-Human CD81 Monoclonal Antibody Unconjugated Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC01281
小鼠 单克隆 (1D6)
反应物种: 人类
应用: 流式细胞仪
规格: 100微克, 500微克
价格: 147美元
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Anti-Human CD81 Monoclonal Antibody APC Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC01281-APC
小鼠 单克隆 (1D6)
反应物种: 人类
共轭标签: APC
应用: 流式细胞仪
规格: 100试验, 200试验
价格: 325美元
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Anti-Human CD81 Monoclonal Antibody FITC Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC01281-FITC
小鼠 单克隆 (1D6)
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 流式细胞仪
规格: 100试验, 200试验
价格: 235美元
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Anti-Human CD81 Monoclonal Antibody PE Conjugated, Flow Validated
武汉博士德生物工程有限公司
目录: FC01281-PE
小鼠 单克隆 (1D6)
反应物种: 人类
共轭标签: PE
应用: 流式细胞仪
规格: 100试验, 200试验
价格: 279美元
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