抗CDK1抗体(单克隆, A17)
武汉博士德生物工程有限公司
目录: MA1077
小鼠 单克隆 (A17)
反应物种: 人类, 小鼠, 大鼠, 鸡
应用: 免疫印迹, 免疫组化

Boster Kit Box
规格: 100μg/vial
价格: 315美元
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抗CDK1抗体(单克隆, PSTAIR)
武汉博士德生物工程有限公司
目录: MA1087
小鼠 单克隆 (PSTAIR)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Boster Kit Box
规格: 100μg/vial
价格: 315美元
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抗细胞周期蛋白依赖性激酶 1 CDK1抗体
武汉博士德生物工程有限公司
目录: PA1544
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Anti-CDK1 antibody, PA1544, Western blotting. Lane 1: HELA Cell Lysate. Lane 2: 293T Cell Lysate. Lane 3: A431 Cell Lysate. Lane 4: CEM Cell Lysate. Lane 5: JURKAT Cell Lysate.
规格: 100μg/vial
价格: 315美元
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Anti-CDK1 Antibody Picoband™
武汉博士德生物工程有限公司
目录: PB9533
domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

Anti- CDK1 Picoband antibody, PB9533, Western blotting. All lanes: Anti CDK1 (PB9533) at 0.5ug/ml. Lane 1: Rat Thymus Tissue Lysate at 50ug. Lane 2: Rat Spleen Tissue Lysate at 50ug. Lane 3: MCF-7 Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Lane 6: NIH3T3 Whole Cell Lysate at 40ug. Predicted bind size: 34KD. Observed bind size: 34KD

Anti- CDK1 Picoband antibody, PB9533, IHC(P). IHC(P): Mouse Testis Tissue

Anti- CDK1 Picoband antibody, PB9533, IHC(P). IHC(P): Rat Testis Tissue
规格: 100μg/vial
价格: 315美元
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抗CDK1/Cdc2兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00209-2
domestic rabbit 单克隆 (BOH-3)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

Immunohistochemical analysis of paraffin-embedded human colon, using CDK1 Antibody(M00209-2). CDK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK1 Antibody (M00209-2)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of CDK1 expression in HeLa cell lysate (M00209-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-2) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
规格: 100微升
价格: 315美元
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抗磷酸化Cdk1/2(T14)兔单克隆抗体
武汉博士德生物工程有限公司
目录: P00209-1
domestic rabbit 单克隆 (DFH-3)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫沉淀

Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody(P00209-1). CDK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDK1 Antibody (P00209-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Phospho-Cdk1/2 (T14) expression in HeLa cell lysate treated with hydroxyurea (P00209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # P00209-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
规格: 100微升
价格: 315美元
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Anti-CDK1 Antibody Picoband™ (monoclonal, 2G11)
武汉博士德生物工程有限公司
目录: M00209-6
小鼠 单克隆 (2G11)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
规格: 100µg/vial
价格: 315美元
至产商
抗CDK1/Cdc2兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00209-1
domestic rabbit 单克隆 (AAD-3)
反应物种: 人类
应用: 免疫印迹, 免疫沉淀

Western blot analysis of CDK1 in HeLa cell lysate (M00209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
规格: 100微升
价格: 315美元
至产商
抗CDK1/Cdc2兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00209-3
domestic rabbit 单克隆 (BOI-3)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹

Western blot analysis of CDK1 expression in HeLa cell lysate (M00209-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-3) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
规格: 100微升
价格: 315美元
至产商
抗CDK1/Cdc2兔单克隆抗体
武汉博士德生物工程有限公司
目录: M00209-4
domestic rabbit 单克隆 (CBB-3)
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

Western blot analysis of CDK1 expression in (1)A431 cell lysate;(2)HeLa cell lysate (M00209-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody (Catalog # M00209-4) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1
规格: 100微升
价格: 315美元
至产商