domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化-石蜡切片
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹, 免疫组化-石蜡切片
Anti-ZEB2 antibody, PA1959, Western blotting. Lane 1: 293T Cell Lysate. Lane 2: HELA Cell Lysate.
Figure 2. IHC analysis of ZEB2 using anti-ZEB2 antibody (PA1959). ZEB2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ZEB2 Antibody (PA1959) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of ZEB2 using anti-ZEB2 antibody (PA1959). ZEB2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ZEB2 Antibody (PA1959) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
Anti- ZEB2antibody, PB9236, Western blotting. All lanes: Anti ZEB2 (PB9236) at 0.5ug/ml. WB: Recombinant Human ZEB2 Protein 0.5ng. Predicted bind size: 45KD. Observed bind size: 45KD
Anti- ZEB2antibody, PB9236, Western blotting. All lanes: Anti ZEB2 (PB9236) at 0.5ug/ml. WB: SMMC Whole Cell Lysate at 40ug. Predicted bind size: 136KD. Observed bind size: 136KD
Figure 3. Flow Cytometry analysis of U937 cells using anti-ZEB2 antibody (PB9236). Overlay histogram showing U937 cells stained with PB9236 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236,1ug/1x10 6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
规格: 100μg/vial
价格: 315美元
至产商
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫组化
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
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