domestic rabbit 多克隆
反应物种: 人类, 牛
应用: 免疫印迹, 免疫组化
反应物种: 人类, 牛
应用: 免疫印迹, 免疫组化
domestic rabbit 单克隆 (IAE-3)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫沉淀, 流式细胞仪
Western blot analysis of CDC42 expression in (1) Jurkat cell lysate; (2) Mouse spleen lysate (M00119). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 monoclonal antibody (Catalog # M00119) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC42
规格: 100微升
价格: 315美元
至产商
domestic rabbit 单克隆 (IBB-3)
反应物种: 人类
应用: 免疫印迹
反应物种: 人类
应用: 免疫印迹
Western blot analysis of Phospho-Rac1/Cdc42 (Ser71) expression in (1) A431 cell lysate treated with LP; (2) A431 cell lysate (P00119). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 monoclonal antibody (Catalog # P00119) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC42
规格: 100微升
价格: 315美元
至产商
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 免疫组化-石蜡切片
Figure 1. IHC analysis of CDC42 using anti-CDC42 antibody (A00119). CDC42 was detected in paraffin-embedded section of human colon cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDC42 Antibody (A00119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of CDC42 using anti-CDC42 antibody (A00119). CDC42 was detected in paraffin-embedded section of human mammary cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDC42 Antibody (A00119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
规格: 100µg/vial
价格: 315美元
至产商
domestic rabbit 多克隆 (polyclonal)
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学
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