产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-beta Catenin/CTNNB1 Picoband Antibody
目录 :
A00004
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 A00004 图像 1
Figure 1. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,. Lane 2: human PC-3 whole cell lysates,. Lane 3: human U-87MG whole cell lysates,. Lane 4: human Caco-2 whole cell lysates,. Lane 5: human Hela whole cell lysates,. Lane 6: human A549 whole cell lysates,. Lane 7: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95KD. The expected band size for CTNNB1 is at 85KD.
图像 2 :
武汉博士德生物工程有限公司 A00004 图像 2
Figure 2. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates,. Lane 2: mouse heart tissue lysates,. Lane 3: mouse lung tissue lysates,. Lane 4: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95KD. The expected band size for CTNNB1 is at 85KD.
图像 3 :
武汉博士德生物工程有限公司 A00004 图像 3
Figure 3. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
A00004
状态 :
Enabled
名称 :
Anti-beta Catenin/CTNNB1 Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
CTNNB1
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for BETA CATENIN/CTNNB1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: ELISA. Reactive species: Human. BETA CATENIN/CTNNB1 information: Subcellular Localization: Cytoplasm. Nucleus; Tissue Specificity: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level).
short description :
Rabbit IgG polyclonal antibody for beta Catenin detection. Tested with WB, IHC-P, ICC/IF, IHC-F, FCM, Direct ELISA in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
CTNNB1: P35222
宿主 :
免疫原 :
E. coli-derived human beta Catenin recombinant protein (Position: A2-K233).
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml. Immunocytochemistry/Immunofluorescence, 2ug/ml. Immunohistochemistry(Frozen Section), 0.5-1ug/mlbr> Flow Cytometry, 1-3ug/1x10 6 cellsbr> Direct ELISA, 0.1-0.5ug/ml.
应用 :
酶联免疫吸附测定, 流式细胞仪, IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Figure 1. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,. Lane 2: human PC-3 whole cell lysates,. Lane 3: human U-87MG whole cell lysates,. Lane 4: human Caco-2 whole cell lysates,. Lane 5: human Hela whole cell lysates,. Lane 6: human A549 whole cell lysates,. Lane 7: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95KD. The expected band size for CTNNB1 is at 85KD. Figure 2. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates,. Lane 2: mouse heart tissue lysates,. Lane 3: mouse lung tissue lysates,. Lane 4: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95KD. The expected band size for CTNNB1 is at 85KD. Figure 3. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 4. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 5. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 6. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 7. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 8. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 9. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 10. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 11. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 12. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 13. IHC analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). CTNNB1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTNNB1 Antibody (A00004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 14. Flow Cytometry analysis of A549 cells using anti-CTNNB1 antibody (A00004). Overlay histogram showing A549 cells stained with A00004 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTNNB1 Antibody (A00004,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Figure 15. IF analysis of CTNNB1 using anti- CTNNB1 antibody (A00004) . CTNNB1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- CTNNB1 Antibody (A00004) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
背景 :
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
研究种类 :
Cancer, Cardiovascular, Cell Adhesion, Cytoplasmic, Cytoskeleton, Cytoskeleton / Ecm, Invasion/Microenvironment, Microfilaments, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Signaling Pathways, Stem Cells, Vascular Inflammation
同义词 :
Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; OK/SW-cl.35, PRO2286;
基因全称 :
catenin beta 1
protein function :
Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).
subcellular localization :
Cytoplasm. Nucleus
组织特异性 :
Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level).
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
更新 :
4/16/19 1:39
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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