产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-BRCA1 Picoband Antibody
目录 :
A00005
规格 :
100 ug/vial
价格 :
240美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 A00005 图像 1
Figure 1. Western blot analysis of BRCA1 using anti- BRCA1 antibody (A00005). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, . Lane 2: SKOV3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCA1 antigen affinity purified polyclonal antibody (Catalog # A00005) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCA1 at approximately 207KD. The expected band size for BRCA1 is at 207KD.
产品信息
SKU号 :
A00005
状态 :
Enabled
名称 :
Anti-BRCA1 Picoband Antibody
目录名称 :
一抗, 多克隆抗体
基因名称 :
乳腺癌1号基因
价格 :
240美元
price various sizes :
30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for BRCA1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. BRCA1 information: Molecular Weight: 207721 MW; Subcellular Localization: Nucleus . Chromosome . Cytoplasm . Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex. Translocated to the cytoplasm during UV-induced apoptosis; Tissue Specificity: Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
short description :
Rabbit IgG polyclonal antibody for Breast cancer type 1 susceptibility protein(BRCA1) detection. Tested with WB in Human.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P38398
宿主 :
免疫原 :
E.coli-derived human BRCA1 recombinant protein (Position: T1681-E1781). Human BRCA1 shares 73.3% and 74.3% amino acid (aa) sequence identity with mouse and rat BRCA1, respectively.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升, 人类
应用 :
免疫印迹
反应物种 :
人类
图像标记 :
Figure 1. Western blot analysis of BRCA1 using anti- BRCA1 antibody (A00005). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, . Lane 2: SKOV3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCA1 antigen affinity purified polyclonal antibody (Catalog # A00005) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCA1 at approximately 207KD. The expected band size for BRCA1 is at 207KD.
背景 :
BRCA1, mapped to 17q21.3, is also known as BRCC1. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by direct mutation or by alterations in gene expression.
competitor equivalent skus :
sc 25259 sc 10466 sc 15372 sc 10467 sc 10469 sc 81416 sc 393608 sc 1021 sc 646 sc 28234 sc 7867 sc 56030 sc 641 sc 642 sc 1553 sc 135732 sc 6954 sc 135731 sc 646 G sc 514640 sc 514797
研究种类 :
Cancer, Cancer Susceptibility, Cell Biology, Chromatin Modifying Enzymes, Dna / Rna, Dna Damage & Repair, Dna Damage Response, Epigenetics And Nuclear Signaling, Oncoproteins/Suppressors, Proteasome / Ubiquitin, Proteolysis / Ubiquitin, Ring Finger E3 Ligase, Transcription, Tumor Suppressors, Ubiquitin E3 Enzymes
同义词 :
Breast cancer type 1 susceptibility protein;6.3.2.-;RING finger protein 53;BRCA1;RNF53;
基因全称 :
Breast cancer type 1 susceptibility protein
分子量 :
207721分子量
protein function :
E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (PubMed:20160719).
subcellular localization :
Nucleus . Chromosome . Cytoplasm . Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex. Translocated to the cytoplasm during UV-induced apoptosis.
组织特异性 :
Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
蛋白名 :
Breast cancer type 1 susceptibility protein
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
更新 :
3/14/19 9:00
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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