产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-CTLA4 Picoband Antibody
目录 :
A00020-1
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 A00020-1 图像 1
Figure 1. Western blot analysis of CTLA4 using anti-CTLA4 antibody (A00020-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTLA4 antigen affinity purified polyclonal antibody (Catalog # A00020-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTLA4 at approximately 25KD. The expected band size for CTLA4 is at 25KD.
图像 2 :
武汉博士德生物工程有限公司 A00020-1 图像 2
Figure 2. IHC analysis of CTLA4 using anti-CTLA4 antibody (A00020-1). . CTLA4 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTLA4 Antibody (A00020-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 A00020-1 图像 3
Figure 3. IHC analysis of CTLA4 using anti-CTLA4 antibody (A00020-1). . CTLA4 was detected in paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTLA4 Antibody (A00020-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
A00020-1
状态 :
Enabled
名称 :
Anti-CTLA4 Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
Ctla4
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
描述 :
Polyclonal antibody for CTLA 4/Ctla4 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: ELISA. Reactive species: Mouse. CTLA 4/Ctla4 information: Molecular Weight: 24993 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein ; Tissue Specificity: Widely expressed with highest levels in lymphoid tissues.
short description :
Rabbit IgG polyclonal antibody for Cytotoxic T-lymphocyte protein 4(Ctla4) detection. Tested with WB, IHC-P, ELISA in Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P09793
宿主 :
免疫原 :
E. coli-derived mouse CTLA4 recombinant protein (Position: E36-D161). Mouse CTLA4 shares 67.5% amino acid (aa) sequence identity with human CTLA4.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml, Mouse. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Mouse, Rat, By Heat. ELISA , 0.1-0.5ug/ml, Mouse.
应用 :
酶联免疫吸附测定, 免疫组化-P, 免疫印迹
反应物种 :
小鼠, 大鼠
图像标记 :
Figure 1. Western blot analysis of CTLA4 using anti-CTLA4 antibody (A00020-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTLA4 antigen affinity purified polyclonal antibody (Catalog # A00020-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTLA4 at approximately 25KD. The expected band size for CTLA4 is at 25KD. Figure 2. IHC analysis of CTLA4 using anti-CTLA4 antibody (A00020-1). . CTLA4 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTLA4 Antibody (A00020-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 3. IHC analysis of CTLA4 using anti-CTLA4 antibody (A00020-1). . CTLA4 was detected in paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTLA4 Antibody (A00020-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 4. IHC analysis of CTLA4 using anti-CTLA4 antibody (A00020-1). . CTLA4 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTLA4 Antibody (A00020-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
背景 :
CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.
competitor equivalent skus :
sc 376016 sc 9094 sc 1628 sc 1630 sc 18829
研究种类 :
T Cells, T Lymphocytic Lineage
同义词 :
细胞毒性T淋巴细胞 蛋白4;细胞毒性T淋巴细胞 抗原4;CTLA-4;CD152;Ctla4;Cd152;
基因全称 :
细胞毒性T淋巴细胞 蛋白4
分子量 :
24993分子量
protein function :
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (By similarity).
subcellular localization :
Cell membrane ; Single-pass type I membrane protein .
组织特异性 :
Widely expressed with highest levels in lymphoid tissues.
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
更新 :
3/27/19 9:01
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits