产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Aromatase/Cyp19a1 Antibody Picoband
目录 :
A00071-2
规格 :
10微克样本
价格 :
99美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
多克隆
反应物种 :
小鼠
应用 :
免疫印迹, 酶联免疫吸附测定
产品信息
SKU号 :
A00071-2
状态 :
1
名称 :
Anti-Aromatase/Cyp19a1 Antibody Picoband
url key :
抗芳香酶cyp19a1
产品种类 :
一抗
基因名称 :
Cyp19a1
价格 :
315
规格1 :
10微克样本
price for size 1 :
99美元
规格2 :
Full size
price for size 2 :
315
规格3 :
Full size carrier free
price for size 3 :
365
克隆性 :
多克隆
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
contents :
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
描述 :
Boster Bio Anti-Aromatase/Cyp19a1 Antibody Picoband catalog # A00071-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
short description :
Boster Bio Anti-Aromatase/Cyp19a1 Antibody Picoband catalog # A00071-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
规格 :
100 ug/vial
UniProt数据库编号 :
P28649
宿主 :
免疫原 :
E.coli-derived mouse Aromatase/Cyp19a1 recombinant protein (Position: N75-Q428).
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year from date of receipt. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for six months. Avoid repeated freezing and thawing.
重组 :
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
应用细节 :
Western blot, 0.25-0.5 ug/ml, Human, Mouse, Rat. Direct ELISA, 0.1-0.5 ug/ml, Mouse.
应用 :
免疫印迹, 酶联免疫吸附测定
反应物种 :
人类, 小鼠, 大鼠
images :
/a/0/a00071-2-cyp19a1-primary-antibodies-wb-testing-1.jpg /a/0/a00071-2-cyp19a1-primary-antibodies-wb-testing-2.jpg
图像标记 :
Figure 1. Western blot analysis of Aromatase/Cyp19a1 using anti-Aromatase/Cyp19a1 antibody (A00071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, . Lane 2: human HepG2 whole cell lysates, . Lane 3: human HCCT tissue lysates, . Lane 4: human HCCP tissue lysates, . Lane 5: human U-87MG whole cell lysates, . Lane 6: human MCF-7 whole cell lysates, . Lane 7: human SK-OV-3 whole cell lysates, . Lane 8: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase/Cyp19a1 antigen affinity purified polyclonal antibody (Catalog # A00071-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase/Cyp19a1 at approximately 49-58 kDa. The expected band size for Aromatase/Cyp19a1 is at 58 kDa. Figure 2. Western blot analysis of Aromatase/Cyp19a1 using anti-Aromatase/Cyp19a1 antibody (A00071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, . Lane 2: rat ovary tissue lysates, . Lane 3: rat brain tissue lysates, . Lane 4: rat C6 whole cell lysates, . Lane 5: mouse testis tissue lysates, . Lane 6: mouse ovary tissue lysates, . Lane 7: mouse brain tissue lysates, . Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase/Cyp19a1 antigen affinity purified polyclonal antibody (Catalog # A00071-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase/Cyp19a1 at approximately 49-58 kDa. The expected band size for Aromatase/Cyp19a1 is at 58 kDa.
custom options :
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created at :
12/1/21 2:59
updated at :
3/2/22 2:57
meta title :
芳香酶/Cyp19a1抗体
meta keyword :
Anti-Aromatase/Cyp19a1 Antibody Picoband
meta description :
Boster Bio Anti-Aromatase/Cyp19a1 Antibody Picoband catalog # A00071-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
应用注释 :
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
背景 :
CYP19A1, also called Aromatase, is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
custom attribute 6 :
Figure 2. Western blot analysis of Aromatase/Cyp19a1 using anti-Aromatase/Cyp19a1 antibody (A00071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, . Lane 2: rat ovary tissue lysates, . Lane 3: rat brain tissue lysates, . Lane 4: rat C6 whole cell lysates, . Lane 5: mouse testis tissue lysates, . Lane 6: mouse ovary tissue lysates, . Lane 7: mouse brain tissue lysates, . Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase/Cyp19a1 antigen affinity purified polyclonal antibody (Catalog # A00071-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase/Cyp19a1 at approximately 49-58 kDa. The expected band size for Aromatase/Cyp19a1 is at 58 kDa.
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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