Anti-IL-1 beta/IL1B Antibody Picoband™ | 武汉博士德生物工程有限公司 A00101 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

Anti-IL-1 beta/IL1B Antibody Picoband™

A00101

规格 :

100 ug/vial

价格 :

315美元

克隆性 :

多克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

反应物种 :

人类, 小鼠, 大鼠

应用 :

免疫印迹

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图像

图像 1 :

Figure 1. Western blot analysis of IL-1 beta using anti-IL-1 beta antibody (A00101). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysate,. Lane 2: rat spleen tissue lysate,. Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 beta antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 beta at approximately 35KD. The expected band size for IL-1 beta is at 31KD.

产品信息

SKU号 :

A00101

产品名称 :

Anti-IL-1 beta/IL1B Antibody Picoband™

价格 :

315美元

规格 :

100 ug/vial

克隆性 :

多克隆

宿主 :

兔

反应物种 :

人类, 小鼠, 大鼠

应用(s) :

免疫印迹

应用细节 :

免疫印迹, 0.1 0.5µg/毫升, 人类, 小鼠, 大鼠

应用注释 :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

描述 :

Boster Bio Anti-IL-1 beta/IL1B Antibody Picoband™ catalog # A00101. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

浓度 :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

基因名称 :

IL1B

UniProt数据库编号 :

P01584

免疫原 :

E. coli-derived human IL-1 beta recombinant protein (Position: A117-S269). Human IL-1 beta shares 78.3% and 77.6% amino acid (aa) sequence identity with mouse and rat IL-1 beta, respectively.

形式 :

冻干

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

纯化 :

免疫原亲和纯化

交叉活性 :

No cross reactivity with other proteins.

储存 :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

重组 :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

基因全称 :

白细胞介素1β

同义词 :

Interleukin-1 beta; IL-1 beta; Catabolin; IL1B; IL1F2;

分子量 :

30748分子量

Protein Function :

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Subcellular Localization :

Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

组织特异性 :

Expressed in activated monocytes/macrophages (at protein level).

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

研究种类 :

Atherosclerosis, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Metabolism, Obesity, Vascular Inflammation

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公司信息