抗体

Anti-Angiogenin/ANG Antibody Picoband™

A00146

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定

Anti-Angiogenin/ANG Antibody Picoband™

315美元

100μg/vial

多克隆

兔

人类

酶联免疫吸附测定, 免疫印迹

酶联免疫吸附测定, 0.1 0.5µg/毫升, 人类免疫印迹, 0.1 0.5µg/毫升, 人类

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Angiogenin/ANG Antibody Picoband™ catalog # A00146. Tested in ELISA, WB applications. This antibody reacts with Human.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

ANG

P03950

E. coli-derived human Angiogenin/ANG recombinant protein (Position: Q25-P147). Human Angiogenin/ANG shares 77.5% amino acid (aa) sequence identity with mouse Angiogenin/ANG.

冻干

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins.

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Angiogenin

Angiogenin; 3.1.27.-; Ribonuclease 5; RNase 5; ANG; RNASE5;

Angiogenin

16550分子量

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.

Nucleus. Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.

Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Angiogenin (Ang), also known as ribonuclease 5, is a small 123 amino acid protein that in humans is encoded by the ANG gene. The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region.

Actin Binding Proteins, Actin Etc, Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cytoskeleton, Cytoskeleton / Ecm, DNA / RNA, Epigenetics and Nuclear Signaling, Hypoxia, Invasion/Microenvironment, Metabolism, Metabolism Processes, Microfilaments, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways And Processes, Response To Hypoxia, RNA Processing, Signal Transduction, Translation