产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Nanog Antibody Picoband™
目录 :
A00153-3
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 流式细胞仪
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
图像
图像 1 :
武汉博士德生物工程有限公司 A00153-3 图像 1
Western blot analysis of Nanog expression in rat ovary extract (lane 1), mouse ovary extract (lane 2) and MCF-7 whole cell lysates (lane 3). Nanog at 48KD was detected using rabbit anti- Nanog Antigen Affinity purified polyclonal antibody (Catalog # A00153-3) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
图像 2 :
武汉博士德生物工程有限公司 A00153-3 图像 2
Figure 2. Flow Cytometry analysis of A549 cells using anti-Nanog antibody (A00153-3). Overlay histogram showing A549 cells stained with A00153-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- Nanog Antibody (A00153-3,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
图像 3 :
武汉博士德生物工程有限公司 A00153-3 图像 3
Figure 3. Flow Cytometry analysis of PC-3 cells using anti-Nanog antibody (A00153-3). Overlay histogram showing PC-3 cells stained with A00153-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- Nanog Antibody (A00153-3,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
A00153-3
产品名称 :
Anti-Nanog Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
流式细胞仪, 免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类, 小鼠, 大鼠。 流式细胞仪, 1-3μg/1x10^6细胞, 人类
应用注释 :
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Nanog Antibody Picoband™ catalog # A00153-3. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
NANOG
UniProt数据库编号 :
Q9H9S0
免疫原 :
different from the related mouse sequence by three amino acids.
QRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRW
QK),
A synthetic peptide corresponding to a sequence in the middle region of human Nanog (115-155aa
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
同源异形盒蛋白NANOG
同义词 :
Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog; NANOG;
蛋白名 :
同源异形盒蛋白NANOG
分子量 :
34620分子量
Protein Function :
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Subcellular Localization :
Nucleus.
组织特异性 :
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
背景 :
NANOG (pron. nanOg) is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. In humans, this protein is encoded by the NANOG gene. It is mapped to 12p13.31. NANOG is thought to be a key factor in maintaining pluripotency. Moreover, NANOG is also thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. The NANOG protein has been found to be a transcriptional activator for the Rex1 promoter, playing a key role in sustaining Rex1 expression. Knockdown of NANOG in embryonic stem cells results in a reduction of Rex1 expression, while forced expression of NANOG stimulates Rex1 expression.
研究种类 :
Cancer, Cell Cycle, Cell Differentiation, ChIP’ing Antibodies, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Intracellular, Nuclear Signaling Pathways, Stem Cells, Surface Molecules, Transcription, Transcription Factors
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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