产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-NADPH oxidase 4/NOX4 Picoband Antibody
目录 :
A00403
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 A00403 图像 1
Figure 1. Western blot analysis of NADPH oxidase 4 using anti-NADPH oxidase 4 antibody (A00403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human MCF-7 whole cell lysates,. Lane 3: human HepG2 whole cell lysates,. Lane 4: human A549 whole cell lysates,. Lane 5: human 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4 antigen affinity purified polyclonal antibody (Catalog # A00403) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NADPH oxidase 4 at approximately 85KD. The expected band size for NADPH oxidase 4 is at 67KD.
图像 2 :
武汉博士德生物工程有限公司 A00403 图像 2
Figure 2. Western blot analysis of NADPH oxidase 4 using anti-NADPH oxidase 4 antibody (A00403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates,. Lane 2: rat NRK whole cell lysates,. Lane 3: mouse kidney tissue lysates,. Lane 4: mouse HEPA1-6 whole cell lysates,. Lane 5: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4 antigen affinity purified polyclonal antibody (Catalog # A00403) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NADPH oxidase 4 at approximately 85KD. The expected band size for NADPH oxidase 4 is at 67KD.
图像 3 :
武汉博士德生物工程有限公司 A00403 图像 3
Figure 3. IHC analysis of NADPH oxidase 4 using anti-NADPH oxidase 4 antibody (A00403). NADPH oxidase 4 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NADPH oxidase 4 Antibody (A00403) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
A00403
状态 :
Enabled
名称 :
Anti-NADPH oxidase 4/NOX4 Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
NOX4
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
描述 :
Polyclonal antibody for NOX4 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. NOX4 information: Subcellular Localization: Endoplasmic reticulum membrane; May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus; Tissue Specificity: Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
short description :
Rabbit IgG polyclonal antibody for NADPH oxidase 4 detection. Tested with WB, IHC-P in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
Q9NPH5
宿主 :
免疫原 :
A合成多肽对应于a序列人的NADPH氧化酶4(ILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLL)
形式 :
冻干
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升。 免疫组化(石蜡包埋切片), 0.5-1微克/毫升
应用 :
免疫组化, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Figure 1. Western blot analysis of NADPH oxidase 4 using anti-NADPH oxidase 4 antibody (A00403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human MCF-7 whole cell lysates,. Lane 3: human HepG2 whole cell lysates,. Lane 4: human A549 whole cell lysates,. Lane 5: human 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4 antigen affinity purified polyclonal antibody (Catalog # A00403) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NADPH oxidase 4 at approximately 85KD. The expected band size for NADPH oxidase 4 is at 67KD. Figure 2. Western blot analysis of NADPH oxidase 4 using anti-NADPH oxidase 4 antibody (A00403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates,. Lane 2: rat NRK whole cell lysates,. Lane 3: mouse kidney tissue lysates,. Lane 4: mouse HEPA1-6 whole cell lysates,. Lane 5: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4 antigen affinity purified polyclonal antibody (Catalog # A00403) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NADPH oxidase 4 at approximately 85KD. The expected band size for NADPH oxidase 4 is at 67KD. Figure 3. IHC analysis of NADPH oxidase 4 using anti-NADPH oxidase 4 antibody (A00403). NADPH oxidase 4 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NADPH oxidase 4 Antibody (A00403) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
背景 :
NADPH oxidase 4 is an enzyme that in humans is encoded by the NOX4 gene, and is a member of the NOX family of NADPH oxidases. This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
研究种类 :
Atherosclerosis, Cardiovascular, Cell Biology, Drug Metabolism, Heart Disease, Metabolic Signaling Pathways, Metabolism, Oxidative Stress, Pathways And Processes, Redox Metabolism, Signal Transduction, Vascular Inflammation
同义词 :
NADPH oxidase 4; Kidney oxidase-1; KOX-1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; NOX4; RENOX
基因全称 :
NADPH氧化酶4
protein function :
Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.
subcellular localization :
Endoplasmic reticulum membrane; May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
组织特异性 :
Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
更新 :
2/6/19 22:57
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits