产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-IGFBP3 Antibody Picoband™
目录 :
A00435-2
规格 :
100 ug/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
图像
图像 1 :
武汉博士德生物工程有限公司 A00435-2 图像 1
Figure 1. Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IGFBP3 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (Catalog # A00435-2) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 37KD. The expected band size for IGFBP3 is at 29KD.
产品信息
SKU号 :
A00435-2
产品名称 :
Anti-IGFBP3 Antibody Picoband™
价格 :
315美元
规格 :
100 ug/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类
应用(s) :
酶联免疫吸附测定, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, human. ELISA (Cap), 1-5µg/ml, human.
应用注释 :
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-IGFBP3 Antibody Picoband™ catalog # A00435-2. Tested in ELISA, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
胰岛素样生长因子结合蛋白3
UniProt数据库编号 :
IGFBP3: P17936
免疫原 :
E. coli-derived human IGFBP3 recombinant protein (Position: A29-K267).
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
胰岛素样生长因子结合蛋白3
同义词 :
胰岛素样生长因子结合蛋白3;IBP-3;IGF结合蛋白3;IGFBP-3;胰岛素样生长因子结合蛋白3;IBP3;
Protein Function :
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.
Subcellular Localization :
Secreted
组织特异性 :
Expressed by most tissues. Present in plasma.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
背景 :
IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
研究种类 :
Angiogenesis, Apoptosis, Associated Proteins, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Diabetes Associated, Growth Factors/Hormones, Metabolism, Obesity, Protein Phosphorylation, Receptors, Signal Transduction, Tyrosine Phosphatases
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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