产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-PPAR gamma/PPARG Antibody Picoband™
目录 :
A00449-2
规格 :
100 ug/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of PPAR gamma using anti-PPAR gamma antibody (A00449-2).
. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysate,. Lane 2: mouse lung tissue lysate,. Lane 3: human A549 whole cell lysate,. Lane 4: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPAR gamma antigen affinity purified polyclonal antibody (Catalog # A00449-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPAR gamma at approximately 67KD. The expected band size for PPAR gamma is at 58KD.
产品信息
SKU号 :
A00449-2
产品名称 :
Anti-PPAR gamma/PPARG Antibody Picoband™
价格 :
315美元
规格 :
100 ug/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
流式细胞仪, 免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类, 小鼠, 大鼠。 流式细胞仪, 1-3μg/1x10^6细胞, 人类
应用注释 :
Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-PPAR gamma/PPARG Antibody Picoband™ catalog # A00449-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
过氧化物酶体增生物激活受体gamma
UniProt数据库编号 :
P37231
免疫原 :
identical to the related mouse and rat sequences.
AIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKH
LYD),
A synthetic peptide corresponding to a sequence in the middle region of human PPAR gamma (207-248aa
AIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKH
LYD),
A synthetic peptide corresponding to a sequence in the middle region of human PPAR gamma (207-248aa
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
过氧化物酶体增殖物激活受体γ
同义词 :
Peroxisome proliferator-activated receptor gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3;
分子量 :
57620分子量
Protein Function :
Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).
Subcellular Localization :
Nucleus. Cytoplasm. Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner. CCRN4L/NOC enhances its nuclear translocation.
组织特异性 :
Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
背景 :
The peroxisome proliferator-activated receptors (PPARs) are a group of three nuclear receptor isoforms, PPAR gamma, PPAR alpha, and PPAR delta, encoded by different genes. PPARs are ligand-regulated transcription factors that control gene expression by binding to specific response elements (PPREs) within promoters. PPAR gamma is a transcription factor that has a pivotal role in adipocyte differentiation and expression of adipocyte-specific genes. The PPAR gamma1 and gamma2 isoforms result from alternative splicing and have ligand-dependent and -independent activation domains. PPAR gamma is a member of a family of nuclear receptors/ligand-dependent transcription factors, which bind to hormone response elements on target gene promoters. PPAR gamma is abundantly expressed in normal lung tissues, especially in endothelial cells, but that its expression is reduced or absent in the angiogenic plexiform lesions of pulmonary hypertensive lungs and in the vascular lesions of a rat model of severe pulmonary hypertension. And it is concluded that fluid shear stress decreases the expression of PPARgamma in endothelial cells and that loss of PPARgamma expression characterizes an abnormal, proliferating, apoptosis-resistant endothelial cell phenotype.
研究种类 :
Atherosclerosis, Cardiovascular, ChIP’ing Antibodies, Diabetes, Diabetes Associated, Domain Families, Epigenetics and Nuclear Signaling, Fatty Acid Oxidation, Fatty Acids, Heart Disease, Lipid And Lipoprotein Metabolism, Lipids / Lipoproteins, Mesenchymal Stem Cells, Metabolic Signaling Pathways, Metabolism, Neurology Process, Neuroscience, Obesity, Pathways And Processes, Redox Metabolism, Stem Cells, Transcription, Zinc Finger
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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