产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗NEMO/IKK丙抗体
目录 :
A00874-1
规格 :
100微克
价格 :
349美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹, 免疫沉淀
更多信息或购买 :
图像
图像 1 :
Figure 2. Western blot analysis of IKBKG using anti-IKBKG antibody (A00874-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKG antigen affinity purified polyclonal antibody (Catalog # A00874-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IKBKG.
图像 2 :
Figure 1. Western blot analysis of IKBKG using anti-IKBKG antibody (A00874-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKG antigen affinity purified polyclonal antibody (Catalog # A00874-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IKBKG.
产品信息
SKU号 :
A00874-1
状态 :
Enabled
名称 :
抗NEMO/IKK丙抗体
目录名称 :
一抗, 多克隆抗体
基因名称 :
IKBKG
价格 :
349美元
price various sizes :
100ug / $349 100ug+Free HRP Secondary BA1054 / $349 100ug+Free Biotin Secondary BA1003 / $349
克隆性 :
多克隆
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
描述 :
Rabbit Polyclonal antibody for IKBKG detection. Tested positive for ELISA,Immunoprecipitation,Western Blot, in human
short description :
Rabbit Polyclonal antibody for IKBKG detection. Tested positive for ELISA,Immunoprecipitation,Western Blot, in human
规格 :
100微克
UniProt数据库编号 :
Q9Y6K9
宿主 :
兔
免疫原 :
Anti-NEMO was affinity purified from whole rabbit serum prepared by repeated immunizations with a recombinant protein of human NEMO.
形式 :
Liquid (sterile filtered)
纯化 :
Anti-NEMO is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody detects human NEMO. Cross reactivity with NEMO from other sources is unknown.
储存 :
Store vial at -20 C prior to opening. Aliquot contents and freeze at -20 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use.
应用细节 :
Western Blot: 1:500-1:1000. ImmunoPrecipitation: 5ug
应用 :
免疫沉淀, 免疫印迹
反应物种 :
人类
图像标记 :
Figure 2. Western blot analysis of IKBKG using anti-IKBKG antibody (A00874-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKG antigen affinity purified polyclonal antibody (Catalog # A00874-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IKBKG. Figure 1. Western blot analysis of IKBKG using anti-IKBKG antibody (A00874-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKBKG antigen affinity purified polyclonal antibody (Catalog # A00874-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IKBKG.
研究种类 :
Apoptosis, Apoptotic Markers, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Epigenetics And Nuclear Signaling, Immunology, Innate Immunity, Intracellular, Nfkb Pathway, Nuclear Signaling, Nuclear Signaling Pathways, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction, Signaling Pathway, Tlr Signaling, Vascular Inflammation
更新 :
2/6/19 22:57
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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