产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-IKK gamma/IKBKG Picoband Antibody
目录 :
A00874
规格 :
100 ug/vial
价格 :
240美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 流式细胞仪
图像
图像 1 :
武汉博士德生物工程有限公司 A00874 图像 1
Figure 1. Western blot analysis of IKK gamma using anti-IKK gamma antibody (A00874). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat cardiac muscle tissue lysates,. lane 2: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK gamma antigen affinity purified polyclonal antibody (Catalog # A00874) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK gamma at approximately 48KD. The expected band size for IKK gamma is at 48KD.
图像 2 :
武汉博士德生物工程有限公司 A00874 图像 2
Figure 2. Flow Cytometry analysis of U87 cells using anti-IKK gamma antibody (A00874). Overlay histogram showing U87 cells stained with A00874 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK gamma Antibody (A00874,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
图像 3 :
武汉博士德生物工程有限公司 A00874 图像 3
Figure 3. Flow Cytometry analysis of PC-3 cells using anti-IKK gamma antibody (A00874). Overlay histogram showing PC-3 cells stained with A00874 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK gamma Antibody (A00874,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
A00874
状态 :
Enabled
名称 :
Anti-IKK gamma/IKBKG Picoband Antibody
目录名称 :
一抗, 多克隆抗体
基因名称 :
IKBKG
价格 :
240美元
price various sizes :
30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240
克隆性 :
多克隆
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
描述 :
Polyclonal antibody for IKK gamma/IKBKG detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. IKK gamma/IKBKG information: Molecular Weight: 48198 MW; Subcellular Localization: Cytoplasm . Nucleus . Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress; Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
short description :
Rabbit IgG polyclonal antibody for NF-kappa-B essential modulator(IKBKG) detection. Tested with WB, FCM in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
Q9Y6K9
宿主 :
免疫原 :
different from the related mouse and rat sequences by three amino acids.
QSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHI
K),
A synthetic peptide corresponding to a sequence in the middle region of human IKK gamma (207-246aa
形式 :
冻干
储存 :
At -20?C for one year. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for a longer time. Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins.
抗体亚型 :
兔IgG
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升, 人类, 小鼠, 大鼠。 流式细胞仪, 1-3微克/1x10 6细胞, 人类
应用 :
流式细胞仪, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Figure 1. Western blot analysis of IKK gamma using anti-IKK gamma antibody (A00874). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat cardiac muscle tissue lysates,. lane 2: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK gamma antigen affinity purified polyclonal antibody (Catalog # A00874) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK gamma at approximately 48KD. The expected band size for IKK gamma is at 48KD. Figure 2. Flow Cytometry analysis of U87 cells using anti-IKK gamma antibody (A00874). Overlay histogram showing U87 cells stained with A00874 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK gamma Antibody (A00874,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Figure 3. Flow Cytometry analysis of PC-3 cells using anti-IKK gamma antibody (A00874). Overlay histogram showing PC-3 cells stained with A00874 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK gamma Antibody (A00874,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
competitor equivalent skus :
sc 53027 sc 25609 sc 1778 sc 9354 sc 19609 sc 52219 sc 69947 sc 69946 sc 15844 sc 15843 sc 15840 sc 8256 sc 8330 sc 166700 sc 166397 sc 166398 sc 8032 sc 56919 sc 52930 sc 71331 sc 8256 R
研究种类 :
Nuclear Signaling Pathways, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction, Signaling Pathway, Tlr Signaling, Vascular Inflammation
同义词 :
NF-kappa-B essential modulator;NEMO;FIP-3;IkB kinase-associated protein 1;IKKAP1;Inhibitor of nuclear factor kappa-B kinase subunit gamma;I-kappa-B kinase subunit gamma;IKK-gamma;IKKG;IkB kinase subunit gamma;NF-kappa-B essential modifier;IKBKG;FIP3, NEMO;
基因全称 :
NF-kappa-B essential modulator
分子量 :
48198分子量
protein function :
Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'- linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B- mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.
subcellular localization :
Cytoplasm . Nucleus . Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress.
组织特异性 :
心, 大脑, 胎盘, 肺, 肝, 骨骼肌, 肾脏和胰腺
recommended detection systems :
Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB.
更新 :
4/1/19 2:52
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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