产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-DYRK1A Antibody Picoband™
目录 :
A00878-1
规格 :
100µg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of DYRK1A using anti-DYRK1A antibody (A00878-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human MCF-7 whole cell lysates,. Lane 3: rat skeletal muscle tissue lysates,. Lane 4: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYRK1A antigen affinity purified polyclonal antibody (Catalog # A00878-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DYRK1A at approximately 100KD. The expected band size for DYRK1A is at 85KD.
图像 2 :
Figure 2. IF analysis of DYRK1A using anti- DYRK1A antibody (A00878-1) . DYRK1A was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- DYRK1A Antibody (A00878-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
图像 3 :
Figure 3. Flow Cytometry analysis of SiHa cells using anti- DYRK1A antibody (A00878-1). Overlay histogram showing SiHa cells stained with A00878-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DYRK1A Antibody (A00878-1,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
A00878-1
产品名称 :
Anti-DYRK1A Antibody Picoband™
价格 :
315美元
规格 :
100µg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
酶联免疫吸附测定, 流式细胞仪, IF, 免疫细胞化学, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml.
Immunocytochemistry/Immunofluorescence, 2µg/ml.
Flow Cytometry, 1-3µg/1x10^6 cells.
Direct ELISA, 0.1-0.5µg/ml.
描述 :
Boster Bio Anti-DYRK1A Antibody Picoband™ catalog # A00878-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
DYRK1A
UniProt数据库编号 :
Q13627
免疫原 :
E. coli-derived human DYRK1A recombinant protein (Position: H38-M269).
形式 :
冻干
Contents :
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
dual specificity tyrosine phosphorylation regulated kinase 1A
同义词 :
Dual specificity tyrosine-phosphorylation-regulated kinase 1A
Protein Function :
Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Exhibits a substrate preference for proline at position P+1 and arginine at position P- 3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (By similarity).
Subcellular Localization :
Nucleus.
组织特异性 :
Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) amd ICC.
背景 :
Dual specificity tyrosine-phosphorylation-regulated kinase 1A is an enzyme that in humans is encoded by the DYRK1A gene. This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms.
研究种类 :
Neurogenesis, Neurology Process, Neuroscience
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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