产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗Orai1抗体
目录 :
A00909
规格 :
100µg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of ORAI1 using anti- ORAI1 antibody (A00909). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, . Lane 2: human Jurka whole cell lysates, . Lane 3: human A549 whole cell lysates, . Lane 4: human A431 whole cell lysates, . Lane 5: human HepG2 whole cell lysates, . Lane 6: human K562 whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ORAI1 antigen affinity purified polyclonal antibody (Catalog # A00909) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORAI1 at approximately 39KD. The expected band size for ORAI1 is at 33KD.
图像 2 :
Figure 2. Flow Cytometry analysis of CACO-2 cells using anti- ORAI1 antibody (A00909). Overlay histogram showing CACO-2 cells stained with A00909 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- ORAI1 Antibody (A00909,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
图像 3 :
Figure 3. Flow Cytometry analysis of A549 cells using anti- ORAI1 antibody (A00909). Overlay histogram showing A549 cells stained with A00909 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- ORAI1 Antibody (A00909,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
A00909
产品名称 :
抗Orai1抗体
价格 :
315美元
规格 :
100µg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
酶联免疫吸附测定, 流式细胞仪, 免疫印迹
应用细节 :
Western blot, 0.25-0.5µg/ml.
Flow Cytometry, 1-3μg/1x10^6 cells. Direct ELISA, 0.1-0.5µg/ml.
应用注释 :
Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Orai1 Antibody catalog # A00909. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
ORAI1
UniProt数据库编号 :
Q96D31
免疫原 :
E.coli-derived human Orai1 recombinant protein (Position: A49-A301).
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
ORAI calcium release-activated calcium modulator 1
同义词 :
Calcium release-activated calcium channel protein 1; Protein orai-1; Transmembrane protein 142A; ORAI1; CRACM1; TMEM142A
Protein Function :
Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
Subcellular Localization :
Cell membrane. Multi-pass membrane protein.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
背景 :
ORAI1 (ORAI calcium release-activated calcium modulator 1), also known as ?CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, FLJ14466, is a calcium selective?ion channel?that in humans is encoded by the ORAI1 gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes severe combined immunodeficiency (SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4 transmembrane domains and form tetramers.?Prakriya et al. showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp (E106D) and glu190-to-gln (E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion.?Prakriya et al. showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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