产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗PDK1(Ab-241)抗体
目录 :
A01159
规格 :
100微升
价格 :
299美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Figure 2. IHC analysis of PDPK1 using anti-PDPK1 antibody (A01159). PDPK1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDPK1 Antibody (A01159) overnight at 4 C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
Figure 1. Western blot analysis of PDPK1 using anti-PDPK1 antibody (A01159). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 antigen affinity purified polyclonal antibody (Catalog # A01159) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PDPK1.
图像 3 :
Immunofluorescence staining of methanol-fixed MCF7 cells using PDK1(Ab-241) Antibody #A01159.
产品信息
SKU号 :
A01159
状态 :
Enabled
名称 :
抗PDK1(Ab-241)抗体
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
PDPK1
价格 :
299美元
price various sizes :
100ul / $299 100ul+Free HRP Secondary BA1054 / $299 100ul+Free Biotin Secondary BA1003 / $299
克隆性 :
多克隆
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
描述 :
Rabbit Polyclonal antibody for O15530 detection. Tested positive for WB IHC IF in Hu Ms Rt
short description :
Rabbit Polyclonal antibody for O15530 detection. Tested positive for WB IHC IF in Hu Ms Rt
规格 :
100微升
UniProt数据库编号 :
O15530
宿主 :
兔
免疫原 :
Peptide sequence around aa.239~243 (A-N-S-F-V) derived from Human PDK1.
形式 :
Liquid
纯化 :
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
储存 :
Store at -20 C for long term preservation (recommended) . Store at 4 C for short term use.
应用细节 :
Predicted MW: 63kd Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
应用 :
IF, 免疫组化, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Figure 2. IHC analysis of PDPK1 using anti-PDPK1 antibody (A01159). PDPK1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDPK1 Antibody (A01159) overnight at 4 C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. Western blot analysis of PDPK1 using anti-PDPK1 antibody (A01159). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 antigen affinity purified polyclonal antibody (Catalog # A01159) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PDPK1. Immunofluorescence staining of methanol-fixed MCF7 cells using PDK1(Ab-241) Antibody #A01159.
研究种类 :
Actin Assembly, Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction
更新 :
2/6/19 22:57
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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