产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Vinculin/VCL Antibody Picoband™
目录 :
A01207
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹
更多信息或购买 :
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图像
图像 1 :
武汉博士德生物工程有限公司 A01207 图像 1
Figure 1. Western blot analysis of Vinculin using anti- Vinculin antibody (A01207). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Vinculin antigen affinity purified polyclonal antibody (Catalog # A01207) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vinculin at approximately 124KD. The expected band size for Vinculin is at 124KD.
产品信息
SKU号 :
A01207
产品名称 :
Anti-Vinculin/VCL Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类, 小鼠, 大鼠
应用注释 :
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Vinculin/VCL Antibody Picoband™ catalog # A01207. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
VCL
UniProt数据库编号 :
P18206
免疫原 :
E.coli-derived human Vinculin recombinant protein (Position: E930-Q1134). Human Vinculin shares 87.7% amino acid (aa) sequence identity with both mouse and rat Vinculin.
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
粘着斑蛋白
同义词 :
Vinculin; Metavinculin; MV; VCL;
蛋白名 :
粘着斑蛋白
分子量 :
123799分子量
Protein Function :
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Subcellular Localization :
Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side. Cell junction, focal adhesion. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity). Colocalizes with LIMD1 in the focal adhesions.
组织特异性 :
Metavinculin is muscle-specific.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
背景 :
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
研究种类 :
Actin Binding Proteins, Actin Etc, Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction
更多信息或购买 :
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公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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