抗大麻受体CB 2抗体 | 武汉博士德生物工程有限公司 A01307 产品信息

产品简要

公司名称 :

武汉博士德生物工程有限公司

产品类型 :

抗体

产品名称 :

抗大麻受体CB 2抗体

A01307

规格 :

100微克

价格 :

299美元

克隆性 :

多克隆

宿主 :

兔

共轭标签 :

nonconjugated

反应物种 :

大鼠

应用 :

免疫印迹, 酶联免疫吸附测定

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图像

图像 1 :

图像 2 :

Figure 1. Western blot analysis of Cnr2 using anti-Cnr2 antibody (A01307). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cnr2 antigen affinity purified polyclonal antibody (Catalog # A01307) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Cnr2.

产品信息

SKU号 :

A01307

状态 :

Enabled

名称 :

抗大麻受体CB 2抗体

目录名称 :

一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体

基因名称 :

Cnr2

价格 :

299美元

price various sizes :

100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299

克隆性 :

多克隆

浓度 :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

共轭标签 :

contents :

Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.

描述 :

Rabbit Polyclonal antibody for Cnr2 detection. Tested positive for ELISA, IHC, WB in Rat

short description :

Rabbit Polyclonal antibody for Cnr2 detection. Tested positive for ELISA, IHC, WB in Rat

规格 :

100微克

UniProt数据库编号 :

Q9QZN9

宿主 :

兔

免疫原 :

Synthetic peptide corresponding to the sequence near the N-terminus of rat cannabinoid receptor CB 2 .

形式 :

Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.

纯化 :

肽亲和纯化

储存 :

-20 C

应用细节 :

Optimal conditions must be determined individually for each application.

应用 :

酶联免疫吸附测定, 免疫印迹

反应物种 :

大鼠

图像标记 :

Membrane ELISA: Lewis rat spleen membranes (5ug/well) were treated with agonist ( 9-THC) and probed with Cannabinoid Receptor (CB2) pAb (1:25 to 1:200 of a 0.1ug/ul stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean SEM (n=2). Figure 1. Western blot analysis of Cnr2 using anti-Cnr2 antibody (A01307). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cnr2 antigen affinity purified polyclonal antibody (Catalog # A01307) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Cnr2.

研究种类 :

Cadherins, Cell Adhesion, Cell Adhesion Proteins, Cytoskeleton / Ecm, Membrane Proteins, Neuroscience, Signal Transduction

更新 :

2/6/19 22:57

更多信息或购买 :

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