产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-DDT Antibody Picoband™
目录 :
A01354
规格 :
100µg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 A01354 图像 1
Figure 1. Western blot analysis of DDT using anti-DDT antibody (A01354). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysate,. Lane 2: rat liver tissue lysates,. Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # A01354) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDT at approximately 14KD. The expected band size for DDT is at 14KD.
图像 2 :
武汉博士德生物工程有限公司 A01354 图像 2
Figure 2. IHC analysis of DDT using anti-DDT antibody (A01354). DDT was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT Antibody (A01354) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 A01354 图像 3
Figure 3. IHC analysis of DDT using anti-DDT antibody (A01354). DDT was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT Antibody (A01354) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
A01354
产品名称 :
Anti-DDT Antibody Picoband™
价格 :
315美元
规格 :
100µg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
流式细胞仪, IF, 免疫组化-P, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升。 免疫组化(石蜡包埋切片), 0.5-1µg/毫升。 免疫细胞化学/免疫荧光, 2µg/毫升。 流式细胞仪, 1-3μg/1x10^6细胞
描述 :
Boster Bio Anti-DDT Antibody Picoband™ catalog # A01354. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
滴滴涕
UniProt数据库编号 :
P30046
免疫原 :
A合成多肽对应于a序列人的滴滴涕(EFLTKELALGQDRILIRFFPLESWQIGKIGTVMTFL)
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
D-dopachrome tautomerase
同义词 :
D-dopachrome decarboxylase; D-dopachrome tautomerase; Phenylpyruvate tautomerase II; DDT
Protein Function :
Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
Subcellular Localization :
细胞质
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
背景 :
DDT, D-dopachrome tautomerization, converts D-dopachrome into 5, 6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.
研究种类 :
细胞生物学
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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