产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Cytokeratin 18/KRT18 Antibody Picoband™
目录 :
A01357-1
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 A01357-1 图像 1
Western blot analysis of Cytokeratin 18 expression in rat liver extract (lane 1), mouse liver extract (lane 2), HELA whole cell lysates (lane 3) and HEPG2 whole cell lysates (lane 4). Cytokeratin 18 at 55KD was detected using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody (Catalog # A01357-1) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
图像 2 :
武汉博士德生物工程有限公司 A01357-1 图像 2
Cytokeratin 18 was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody (Catalog # A01357-1) at 1 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
图像 3 :
武汉博士德生物工程有限公司 A01357-1 图像 3
Cytokeratin 18 was detected in paraffin-embedded sections of mouse kidney tissues using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody (Catalog # A01357-1) at 1 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
产品信息
SKU号 :
A01357-1
产品名称 :
Anti-Cytokeratin 18/KRT18 Antibody Picoband™
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
流式细胞仪, IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Immunohistochemistry(Frozen Section), 0.5-1 μg/ml, Human. Immunocytochemistry, 0.5-1 μg/ml, Human. Immunofluorescence, 2 μg/ml, Human. Flow Cytometry, 1-3 μg/1x10^6 cells, Human.
应用注释 :
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Cytokeratin 18/KRT18 Antibody Picoband™ catalog # A01357-1. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
KRT18
UniProt数据库编号 :
P05783
免疫原 :
E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430). Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Keratin, type I cytoskeletal 18
同义词 :
Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18; KRT18; CYK18; PIG46;
蛋白名 :
Keratin, type I cytoskeletal 18
分子量 :
48058分子量
Protein Function :
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Subcellular Localization :
Cytoplasm, perinuclear region. Nucleus, nucleolus.
组织特异性 :
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
背景 :
Keratin 18, mapped to 12q13.13, is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
研究种类 :
Cell Type Markers, Class I, Cytoskeleton, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Intermediate Filaments, Microtubules, Signal Transduction, Structures, Tumor Associated
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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