产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-LIF Antibody Picoband™
目录 :
A01400
规格 :
100µg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
图像
图像 1 :
武汉博士德生物工程有限公司 A01400 图像 1
Figure 1. Western blot analysis of LIF using anti-LIF antibody (A01400). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: rat heart tissue lysates,. Lane 3: rat liver tissue lysates,. Lane 4: rat kidney tissue lysates,. Lane 5: human Hela cell lysates, . Lane 6: human placenta tissue lysates,. Lane 7: human Jurkat cell lysates, . Lane 8: mouse liver tissue lysates. Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIF antigen affinity purified polyclonal antibody (Catalog # A01400) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIF at approximately 18KD. The expected band size for LIF is at 22KD.
产品信息
SKU号 :
A01400
产品名称 :
Anti-LIF Antibody Picoband™
价格 :
315美元
规格 :
100µg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
酶联免疫吸附测定, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml. ELISA (Cap), 1-5µg/ml.
描述 :
Boster Bio Anti-LIF Antibody Picoband™ catalog # A01400. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
Lif
UniProt数据库编号 :
P09056
免疫原 :
E. coli-derived mouse LIF recombinant protein (Position: S24-F203).
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
leukemia inhibitory factor
同义词 :
Leukemia inhibitory factor; LIF; Differentiation-stimulating factor; D factor; Lif
Protein Function :
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Subcellular Localization :
Secreted.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
背景 :
LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.
研究种类 :
Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Differentiation, Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Neurogenesis, Neurology Process, Neuroscience, Transcription, Tumor Suppressors
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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