产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-CD16/FCGR3A Antibody Picoband™
目录 :
A01408-1
规格 :
100µg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
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图像
图像 1 :
Figure 1. Western blot analysis of CD16 using anti-CD16 antibody (A01408-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates,. Lane 2: mouse HEPA1-6 whole cell lysates,. Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD16 antigen affinity purified polyclonal antibody (Catalog # A01408-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD16 at approximately 45KD. The expected band size for CD16 is at 29KD.
产品信息
SKU号 :
A01408-1
产品名称 :
Anti-CD16/FCGR3A Antibody Picoband™
价格 :
315美元
规格 :
100µg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类
应用(s) :
酶联免疫吸附测定, 流式细胞仪, IF, 免疫组化-P, 免疫细胞化学
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml.
Immunocytochemistry/Immunofluorescence, 5µg/ml.
Flow Cytometry, 1-3μg/1x10^6 cells.
Direct ELISA, 0.1-0.5µg/ml.
描述 :
Boster Bio Anti-CD16/FCGR3A Antibody Picoband™ catalog # A01408-1. Tested in Direct ELISA, FCM, ICC/IF, IHC-P applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
FCGR3A
UniProt数据库编号 :
P08637
免疫原 :
E. coli-derived human CD16 recombinant protein (Position: Q101-D166).
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Fc fragment of IgG receptor IIIa
同义词 :
Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3
Protein Function :
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Subcellular Localization :
Cell membrane; Exists also as a soluble receptor.
组织特异性 :
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
Recommended Detection Systems :
Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
背景 :
Low affinity immunoglobulin gamma Fc region receptor III-A is a protein that in humans is encoded by the FCGR3A gene. This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
研究种类 :
Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Nk Cells, Stem Cells
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公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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