产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-strogen Related Receptor gamma/ESRRG Antibody Picoband‚Ñ¢
目录 :
A01470-1
规格 :
10微克样本
价格 :
99美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 A01470-1 图像 1
Figure 1. Western blot analysis of ESRRG using anti-ESRRG antibody (A01470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, . Lane 2: human 22RV1 whole cell lysates, . Lane 3: human COLO-320 whole cell lysates, . Lane 4: human SW620 whole cell lysates, . Lane 5: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESRRG antigen affinity purified polyclonal antibody (Catalog # A01470-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESRRG at approximately 51KD. The expected band size for ESRRG is at 51KD.
图像 2 :
武汉博士德生物工程有限公司 A01470-1 图像 2
Figure 2. IHC analysis of ESRRG using anti-ESRRG antibody (A01470-1). ESRRG was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 A01470-1 图像 3
Figure 3. IHC analysis of ESRRG using anti-ESRRG antibody (A01470-1). ESRRG was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
A01470-1
状态 :
1
名称 :
Anti-strogen Related Receptor gamma/ESRRG Antibody Picoband‚Ñ¢
url key :
anti-esrrg-picoband-trade-antibody-a01470-1-boster
产品种类 :
一抗
基因名称 :
ESRRG
价格 :
315
规格1 :
10微克样本
price for size 1 :
99美元
规格2 :
Full size
price for size 2 :
315
规格3 :
Full size carrier free
price for size 3 :
365
克隆性 :
多克隆
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
描述 :
Boster Bio Anti-strogen Related Receptor gamma/ESRRG Antibody Picoband catalog # A01470-1. Tested in ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
short description :
Boster Bio Anti-strogen Related Receptor gamma/ESRRG Antibody Picoband catalog # A01470-1. Tested in ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
规格 :
100ug/vial
UniProt数据库编号 :
P62508
宿主 :
免疫原 :
E. coli-derived human ESRRG recombinant protein (Position: Q302-V458).
形式 :
冻干
储存 :
Store at -20ÀöC for one year from date of receipt. After reconstitution, at 4ÀöC for one month. It can also be aliquotted and stored frozen at -20ÀöC for six months. Avoid repeated freeze-thaw cycles.
交叉活性 :
No cross reactivity with other proteins.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml. Immunohistochemistry (Frozen Section), 0.5-1ug/ml. Immunocytochemistry/Immunofluorescence, 2ug/ml. Flow Cytometry, 1-3ug/1x10 6 cells. Direct ELISA, 0.1-0.5ug/ml.
应用 :
酶联免疫吸附测定, 流式细胞仪, IF, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
images :
/A/0/A01470-1-ESRRG-primary-antibodies-IHC-testing-2.jpg /A/0/A01470-1-ESRRG-primary-antibodies-IHC-testing-3.jpg /A/0/A01470-1-ESRRG-primary-antibodies-WB-testing-1.jpg /a/0/a01470-1-esrrg-primary-antibodies-fc-testing-5.png /a/0/a01470-1-esrrg-primary-antibodies-if-testing-4.png
图像标记 :
Figure 2. IHC analysis of ESRRG using anti-ESRRG antibody (A01470-1). ESRRG was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 3. IHC analysis of ESRRG using anti-ESRRG antibody (A01470-1). ESRRG was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 1. Western blot analysis of ESRRG using anti-ESRRG antibody (A01470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates . Lane 2: human 22RV1 whole cell lysates . Lane 3: human COLO-320 whole cell lysates . Lane 4: human SW620 whole cell lysates . Lane 5: mouse SP20 whole cell lysates. After Electrophoresis proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESRRG antigen affinity purified polyclonal antibody (Catalog # A01470-1) at 0.5 μg/mL overnight at 4°C then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESRRG at approximately 51KD. The expected band size for ESRRG is at 51KD. Figure 5. Flow Cytometry analysis of U20S cells using anti-ESRRG antibody (A01470-1). Overlay histogram showing U20S cells stained with A01470-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ESRRG Antibody (A01470-1 1μg/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127 5-10μg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Figure 4. IF analysis of ESRRG using anti-ESRRG antibody (A01470-1). ESRRG was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ESRRG Antibody (A01470-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
custom options :
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created at :
9/22/17 10:41
updated at :
3/2/22 2:55
meta title :
strogen Related Receptor gamma/ESRRG Antibody
meta keyword :
Anti-strogen Related Receptor gamma/ESRRG Antibody Picoband
meta description :
Boster Bio Anti-strogen Related Receptor gamma/ESRRG Antibody Picoband catalog # A01470-1. Tested in ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
rating value :
100
背景 :
Estrogen-related receptor gamma (ERR-gamma), also known as NR3B3, is a nuclear receptor that in humans is encoded by the ESRRG gene. This gene encodes a member of the estrogen receptor-related receptor (ESRR) family, which belongs to the nuclear hormone receptor superfamily. All members of the ESRR family share an almost identical DNA binding domain, which is composed of two C4-type zinc finger motifs. The ESRR members are orphan nuclear receptors; they bind to the estrogen response element and steroidogenic factor 1 response element, and activate genes controlled by both response elements in the absence of any ligands. The ESRR family is closely related to the estrogen receptor (ER) family. They share target genes, co-regulators and promoters, and by targeting the same set of genes, the ESRRs seem to interfere with the ER-mediated estrogen response in various ways. It has been reported that the family member encoded by this gene functions as a transcriptional activator of DNA cytosine-5-methyltransferases 1 (Dnmt1) expression by direct binding to its response elements in the DNMT1 promoters, modulates cell proliferation and estrogen signaling in breast cancer, and negatively regulates bone morphogenetic protein 2-induced osteoblast differentiation and bone formation. Multiple alternatively spliced transcript variants have been identified, which mainly differ at the 5' end and some of which encode protein isoforms differing in the N-terminal region.
研究种类 :
Cancer,Domain Families,Epigenetics And Nuclear Signaling,Nuclear Hormone Receptors,Nuclear Receptors,Nuclear Signaling Pathways,Signal Transduction,Signaling Pathway,Transcription,Zinc Finger
同义词 :
Estrogen-related receptor gamma; ERR gamma-2; Estrogen receptor-related protein 3; Nuclear receptor subfamily 3 group B member 3; ESRRG; ERR3; ERRG2; KIAA0832; NR3B3
基因全称 :
estrogen related receptor gamma
protein function :
Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements (By similarity). Induces the expression of PERM1 in the skeletal muscle.
subcellular localization :
Nucleus.
组织特异性 :
Expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland.
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
template :
抗体
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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