产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Neurexin 1/NRXN1 Picoband Antibody
目录 :
A01490-1
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
图像
图像 1 :
武汉博士德生物工程有限公司 A01490-1 图像 1
Figure 1. Western blot analysis of Neurexin 1 using anti-Neurexin 1 antibody (A01490-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, . Lane 2: human SHG-4 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurexin 1 antigen affinity purified polyclonal antibody (Catalog # A01490-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurexin 1 at approximately 170KD. The expected band size for Neurexin 1 is at 162KD.
产品信息
SKU号 :
A01490-1
状态 :
Enabled
名称 :
Anti-Neurexin 1/NRXN1 Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
NRXN1
价格 :
280美元
price various sizes :
Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
描述 :
Rabbit IgG polyclonal antibody for Neurexin 1 detection. Tested with WB, IHC-F, ICC, FCM, Direct ELISA in Human;Mouse;Rat.
short description :
Rabbit IgG polyclonal antibody for Neurexin 1 detection. Tested with WB, IHC-F, ICC, FCM, Direct ELISA in Human;Mouse;Rat.
规格 :
100 ug/vial
UniProt数据库编号 :
P58400
宿主 :
免疫原 :
E. coli-derived human Neurexin 1 recombinant protein (Position: D141-E294).
形式 :
冻干
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用 :
酶联免疫吸附测定, 流式细胞仪, 免疫组化, 免疫细胞化学, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Figure 1. Western blot analysis of Neurexin 1 using anti-Neurexin 1 antibody (A01490-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, . Lane 2: human SHG-4 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurexin 1 antigen affinity purified polyclonal antibody (Catalog # A01490-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurexin 1 at approximately 170KD. The expected band size for Neurexin 1 is at 162KD. Figure 2. Flow Cytometry analysis of U251 cells using anti-NRXN1 antibody (A01490-1). Overlay histogram showing U251 cells stained with A01490-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRXN1 Antibody (A01490-1,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
背景 :
Neurexin-1-alpha is a protein that in humans is encoded by the NRXN1 gene. This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are cell-surface receptors that bind neuroligins to form Ca(2+)-dependent neurexin/neuroligin complexes at synapses in the central nervous system. This complex is required for efficient neurotransmission and is involved in the formation of synaptic contacts. Three members of this gene family have been studied in detail and are estimated to generate over 3,000 variants through the use of two alternative promoters (alpha and beta) and extensive alternative splicing in each family member. Recently, a third promoter (gamma) was identified for this gene in the 3' region. Mutations in this gene are associated with Pitt-Hopkins-like syndrome-2 and may contribute to susceptibility to schizophrenia.
研究种类 :
Adapters, Axonal Guidance Proteins, Cell Adhesion Proteins, Cell Type Marker, Growth And Development, Membrane Proteins, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction, Synapse Marker, Transmembrane
同义词 :
Neurexin-1-beta; Neurexin I-beta; NRXN1
基因全称 :
neurexin 1
protein function :
Neuronal cell surface protein that may be involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins. May play a role in formation or maintenance of synaptic junctions. May mediate intracellular signaling. May play a role in angiogenesis (By similarity).
subcellular localization :
Cell membrane; Localized on the pre- synaptic membrane.
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(F) and ICC.
更新 :
4/1/19 3:33
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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