产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗eNOS(Ab-1177)抗体
目录 :
A01604-1
规格 :
100微升
价格 :
299美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学
图像
图像 1 :
武汉博士德生物工程有限公司 A01604-1 图像 1
Figure 1. Western blot analysis of NOS3 using anti-NOS3 antibody (A01604-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS3 antigen affinity purified polyclonal antibody (Catalog # A01604-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NOS3.
图像 2 :
武汉博士德生物工程有限公司 A01604-1 图像 2
Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Ab-1177) Antibody #A01604-1.
产品信息
SKU号 :
A01604-1
状态 :
Enabled
名称 :
抗eNOS(Ab-1177)抗体
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
内皮细胞一氧化氮合酶
价格 :
299美元
price various sizes :
100ul / $299 100ul+Free HRP Secondary BA1054 / $299 100ul+Free Biotin Secondary BA1003 / $299
克隆性 :
多克隆
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
描述 :
Rabbit Polyclonal antibody for P29474 detection. Tested positive for WB IF in Hu Ms Rt
short description :
Rabbit Polyclonal antibody for P29474 detection. Tested positive for WB IF in Hu Ms Rt
规格 :
100微升
UniProt数据库编号 :
P29474
宿主 :
免疫原 :
Peptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS.
形式 :
Liquid
纯化 :
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
储存 :
Store at -20 C for long term preservation (recommended) . Store at 4 C for short term use.
应用细节 :
Predicted MW: 140kd Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
应用 :
IF, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Figure 1. Western blot analysis of NOS3 using anti-NOS3 antibody (A01604-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS3 antigen affinity purified polyclonal antibody (Catalog # A01604-1) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NOS3. Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Ab-1177) Antibody #A01604-1.
研究种类 :
Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Metabolism, Metabolism Processes, Neuroscience, Neurotransmission, Oxidative Stress, Pathways And Processes, Platelets, Redox Metabolism, Response To Hypoxia, Vasculature
更新 :
2/6/19 22:57
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits