产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-CD1b Antibody Picoband™
目录 :
A02158-1
规格 :
100µg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 A02158-1 图像 1
Figure 1. Western blot analysis of CD1b using anti-CD1b antibody (A02158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,. Lane 2: human PC-3 whole cell lysate,. Lane 3: human SW620 whole cell lysate,. Lane 4: human THP-1 whole cell lysate,. Lane 5: human MDA-MB-231 whole cell lysate,. Lane 6: human K562 whole cell lysate,. Lane 7: human A431 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1b antigen affinity purified polyclonal antibody (Catalog # A02158-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1b at approximately 65KD. The expected band size for CD1b is at 37KD.
图像 2 :
武汉博士德生物工程有限公司 A02158-1 图像 2
Figure 2. Western blot analysis of CD1b using anti-CD1b antibody (A02158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: rat heart tissue lysates,. Lane 3: rat lung tissue lysates,. Lane 4: mouse brain tissue lysates,. Lane 5: mouse lung tissue lysates,. Lane 6: mouse Neuro-2a whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1b antigen affinity purified polyclonal antibody (Catalog # A02158-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1b at approximately 65KD. The expected band size for CD1b is at 37KD.
图像 3 :
武汉博士德生物工程有限公司 A02158-1 图像 3
Figure 3. IHC analysis of CD1b using anti-CD1b antibody (A02158-1). CD1b was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD1b Antibody (A02158-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
A02158-1
产品名称 :
Anti-CD1b Antibody Picoband™
价格 :
315美元
规格 :
100µg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类
应用(s) :
免疫组化, 免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升。 免疫组化(石蜡包埋切片), 0.5-1µg/毫升
描述 :
Boster Bio Anti-CD1b Antibody Picoband™ catalog # A02158-1. Tested in IHC, WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
CD1B
UniProt数据库编号 :
P29016
免疫原 :
A合成多肽对应于a序列人的CD1b(DKEVAELEEIF RVYIFGFAREVQDFAGDFQMKY)
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
基因全称 :
CD1b分子
同义词 :
T细胞表面的糖蛋白CD1b;CD1b;CD1B
Protein Function :
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Subcellular Localization :
细胞膜
组织特异性 :
Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
背景 :
CD1B is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.
研究种类 :
Adaptive Immunity, B Cells, Cell Type Markers, Immunology, Non-Cd
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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