产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-TPA Tissue Plasminogen Activator/PLAT Picoband Antibody
目录 :
A02965-1
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 A02965-1 图像 1
Figure 1. Western blot analysis of PLAT using anti-PLAT antibody (A02965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human U2OS whole cell lysates,. Lane 3: human U-87MG whole cell lysates,. Lane 4: rat liver tissue lysates,. Lane 5: rat kidney tissue lysates,. Lane 6: mouse liver tissue lysates,. Lane 7: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAT antigen affinity purified polyclonal antibody (Catalog # A02965-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAT at approximately 55-63KD. The expected band size for PLAT is at 63KD.
图像 2 :
武汉博士德生物工程有限公司 A02965-1 图像 2
Figure 2. IHC analysis of PLAT using anti-PLAT antibody (A02965-1). PLAT was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PLAT Antibody (A02965-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 A02965-1 图像 3
Figure 3. Flow Cytometry analysis of U251 cells using anti-PLAT antibody (A02965-1). Overlay histogram showing U251 cells stained with A02965-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLAT Antibody (A02965-1,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
A02965-1
状态 :
Enabled
名称 :
Anti-TPA Tissue Plasminogen Activator/PLAT Picoband Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
PLAT
价格 :
280美元
克隆性 :
多克隆
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
描述 :
Rabbit IgG polyclonal antibody for PLAT detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat.
short description :
Rabbit IgG polyclonal antibody for PLAT detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat.
规格 :
100 ug/vial
UniProt数据库编号 :
P00750
宿主 :
免疫原 :
A合成多肽对应于a序列人的PLAT(QKDVPGVYTKVTNYLDWIRDNMRP)
形式 :
冻干
储存 :
At -20 for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升。 免疫组化(石蜡包埋切片), 0.5-1微克/毫升。 免疫组化(冰冻切片), 0.5-1微克/毫升。 免疫细胞化学, 0.5-1微克/毫升。 流式细胞仪, 1-3微克/1x10 6细胞
应用 :
流式细胞仪, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Figure 1. Western blot analysis of PLAT using anti-PLAT antibody (A02965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human U2OS whole cell lysates,. Lane 3: human U-87MG whole cell lysates,. Lane 4: rat liver tissue lysates,. Lane 5: rat kidney tissue lysates,. Lane 6: mouse liver tissue lysates,. Lane 7: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAT antigen affinity purified polyclonal antibody (Catalog # A02965-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAT at approximately 55-63KD. The expected band size for PLAT is at 63KD. Figure 2. IHC analysis of PLAT using anti-PLAT antibody (A02965-1). PLAT was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PLAT Antibody (A02965-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 3. Flow Cytometry analysis of U251 cells using anti-PLAT antibody (A02965-1). Overlay histogram showing U251 cells stained with A02965-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLAT Antibody (A02965-1,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
背景 :
PLAT is also known as tPA. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
同义词 :
Tissue-type plasminogen activator; t-PA; t-plasminogen activator; tPA; Alteplase; Reteplase; PLAT
基因全称 :
纤溶酶原激活物, 组织类型
protein function :
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration.
subcellular localization :
细胞外间隙
组织特异性 :
Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
更新 :
3/26/19 7:26
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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