抗体

Anti-Tubulin alpha Antibody Picoband™

A03989-1

100µg/vial

315美元

多克隆

domestic rabbit

未共轭

多克隆

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Anti-Tubulin alpha Antibody Picoband™

315美元

100µg/vial

多克隆

兔

人类, 小鼠, 大鼠

免疫印迹, 免疫组化-P, 免疫细胞化学, IF, 流式细胞仪, 酶联免疫吸附测定

Western blot, 0.1-0.25µg/ml, Human, Mouse, Rat. Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human. Immunocytochemistry/Immunofluorescence, 5µg/ml, Human, Rat. Flow Cytometry, 1-3μg/1x10^6 cells, Human. Direct ELISA, 0.1-0.5µg/ml, Human.

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Tubulin alpha Antibody Picoband™ catalog # A03989-1. Tested in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

TUBA1A/TUBA1B/TUBA1C

Q71U36

E.coli-derived human Tubulin alpha recombinant protein (Position: N18-A403).

冻干

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

免疫原亲和纯化

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.