产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-CD84 Antibody Picoband™
目录 :
A04872-2
规格 :
100µg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
Figure 1. Flow Cytometry analysis of THP-1 cells using anti- CD84 antibody (A04872-2). Overlay histogram showing THP-1 cells stained with A04872-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD84 Antibody (A04872-2, 1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
图像 2 :
Figure 2. Flow Cytometry analysis of JK cells using anti- CD84 antibody (A04872-2). Overlay histogram showing JK cells stained with A04872-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD84 Antibody (A04872-2, 1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
A04872-2
产品名称 :
Anti-CD84 Antibody Picoband™
价格 :
315美元
规格 :
100µg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类
应用(s) :
酶联免疫吸附测定, 流式细胞仪, 免疫组化, 免疫细胞化学
应用细节 :
免疫组化(冰冻切片), 0.5-1µg/毫升。 免疫细胞化学, 0.5-1µg/毫升。 流式细胞仪, 1-3µg/1x10^6细胞。 酶联免疫吸附测定, 0.1 0.5µg/毫升
描述 :
Boster Bio Anti-CD84 Antibody Picoband™ catalog # A04872-2. Tested in ELISA, Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
CD84
UniProt数据库编号 :
Q9UIB8
免疫原 :
E. coli-derived human CD84 recombinant protein (Position: K22-G225).
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .
交叉活性 :
No cross reactivity with other proteins.
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
CD84分子
同义词 :
SLAM family member 5; Cell surface antigen MAX.3; Hly9-beta; Leukocyte differentiation antigen CD84; Signaling lymphocytic activation molecule 5; CD84; SLAMF5
Protein Function :
Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Can mediate natural killer (NK) cell cytotoxicity dependent on SH2D1A and SH2D1B (By similarity). Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seem be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A-dependent pathway. May serve as a marker for hematopoietic progenitor cells (PubMed:11564780, PubMed:12115647. PubMed:12928397, PubMed:12962726, PubMed:16037392) Required for a prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation. In germinal centers involved in maintaining B-cell tolerance and in preventing autoimmunity (By similarity). In mast cells negatively regulates high affinity immunoglobulin epsilon receptor signaling; independent of SH2D1A and SH2D1B but implicating FES and PTPN6/SHP-1 (PubMed:22068234). In macrophages enhances LPS-induced MAPK phosphorylation and NF-kappaB activation and modulates LPS-induced cytokine secretion; involving ITSM 2.
Subcellular Localization :
细胞膜。 膜蛋白
组织特异性 :
Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells. Expressed in mast cells.
Recommended Detection Systems :
Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(F) and ICC.
背景 :
CD84 (Cluster of Differentiation 84) is a human protein encoded by the CD84 gene. This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants. Members of the CD2 subgroup of the Ig superfamily, such as CD84, have similar patterns of conserved disulfide bonds and function in adhesion interactions between T lymphocytes and accessory cells.
研究种类 :
Adaptive Immunity, B Cells, Hematopoietic Progenitors, Immunology, Stem Cells, Surface Molecules
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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