抗体

Anti-HERC5 Antibody Picoband™

A05285-2

100µg/vial

315美元

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

Anti-HERC5 Antibody Picoband™

315美元

100µg/vial

多克隆

兔

人类

酶联免疫吸附测定, IF, 免疫细胞化学, 免疫印迹

Western blot, 0.1-0.5µg/ml. Immunocytochemistry/Immunofluorescence, 2µg/ml. Direct ELISA, 0.1-0.5µg/ml.

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-HERC5 Antibody Picoband™ catalog # A05285-2. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

HERC5

Q9UII4

E.coli-derived human HERC5 recombinant protein (Position: F793-G1024).

冻干

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins.

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

HECT和RLD区域含E3泛素蛋白连接酶 5

E3 ISG15--protein ligase HERC5; Cyclin-E-binding protein 1; HECT domain and RCC1-like domain-containing protein 5; HERC5; CEB1; CEBP1

Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.

Perinuclear region.

Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC.

Probable E3 ubiquitin-protein ligase HERC5 is an enzyme that in humans is encoded by the HERC5 gene. It is mapped to 4q22.1. This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.

Cell Biology, Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Interferons, Proteasome / Ubiquitin, Proteolysis / Ubiquitin, Ubiquitin & Ubiquitin Like Modifiers, Ubiquitin E3 Enzymes