产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Rat Activin A PicoKine ELISA Kit
目录 :
EK0303
规格 :
96 wells/kit, with removable strips.
价格 :
320美元
文章摘录数: 1
参考文献
Buzzard J, Loveland K, O Bryan M, O Connor A, Bakker M, Hayashi T, et al. Changes in circulating and testicular levels of inhibin A and B and activin A during postnatal development in the rat. Endocrinology. 2004;145:3532-41 pubmed
图像
图像 1 :
武汉博士德生物工程有限公司 EK0303 图像 1
Rat Activin A PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0303
状态 :
Enabled
名称 :
Rat Activin A PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
INHBA
价格 :
320美元
试验范围 :
15.6pg/毫升1000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Rat Activin A/INHBA with <12pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA) and saliva. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Activin A/INHBA tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Activin A. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P18331
宿主 :
No
免疫原 :
Expression system for standard: CHO; Immunogen sequence: G311-S426
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<12pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, serum, plasma(heparin, EDTA) and saliva.
应用 :
酶联免疫吸附测定
反应物种 :
大鼠
前体活性 :
Bovine, Monkey, Rabbit
图像标记 :
Rat Activin A PicoKine ELISA Kit standard curve
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升, 15.6pg/毫升
背景 :
Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
研究种类 :
Cancer, Growth Factors/Hormones, Signal Transduction, Tumor Biomarkers
同义词 :
Inhibin beta A chain;Activin beta-A chain;Inhba;
基因全称 :
抑制素乙A链
分子量 :
47406分子量
protein function :
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
subcellular localization :
Secreted .
蛋白名 :
抑制素乙A链
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 31 151 450 28 138 453 Standard deviation 1.30 8.00 24.20 1.56 8.69 25.36 CV(%) 4.2% 5.3% 5.6% 5.6% 6.3% 5.6%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 31 27 31 30 1.73 5.7% Sample 2 151 165 144 171 157 10.75 6.8% Sample 3 450 412 404 462 432 24.53 5.6% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.081 0.120 0.222 0.433 0.761 1.429 2.365
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠单克隆抗体来自小鼠
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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