产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human Amphiregulin(AR) / AREG PicoKine ELISA Kit
目录 :
EK0304
规格 :
96 wells/kit, with removable strips.
价格 :
369美元
文章摘录数: 2
参考文献
PETERSON E, Pectasides E, Shabbeer S, Wiechmann L, Sparano J, Kenny P. Evaluation of serum Amphiregulin levels in breast cancer patients and cancer-free controls. Exp Hematol Oncol. 2013;2:25 pubmed 出版商
Liu N, Ma Y, Li R, Jin H, Li M, Huang X, et al. Comparison of follicular fluid amphiregulin and EGF concentrations in patients undergoing IVF with different stimulation protocols. Endocrine. 2012;42:708-16 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0304 图像 1
Human Amphiregulin(AR) PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0304
状态 :
Enabled
名称 :
Human Amphiregulin(AR) / AREG PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
AREG
价格 :
369美元
试验范围 :
15.6pg/毫升1000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human Amphiregulin/AREG with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA), saliva and human milk. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Amphiregulin/AREG tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Amphiregulin(AR). 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P15514
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: S101-K198
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<10pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, serum, plasma(heparin, EDTA), saliva and human milk.
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human Amphiregulin(AR) PicoKine ELISA Kit standard curve
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升, 15.6pg/毫升
背景 :
Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor(EGF) and transforming growth factor alpha(TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.
研究种类 :
Cancer, Growth Factors, Growth Factors/Hormones, Signal Transduction
同义词 :
Amphiregulin;AR;Colorectum cell-derived growth factor;CRDGF;AREG;SDGF;AREGB;
基因全称 :
Amphiregulin
分子量 :
27895分子量
protein function :
Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
subcellular localization :
Membrane; Single-pass membrane protein.
蛋白名 :
Amphiregulin
sequence similarities :
Belongs to the amphiregulin family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 28 200 441 30 197 433 Standard deviation 1.65 10.4 29.54 2.19 10.63 31.6 CV(%) 6.7%% 5.2% 6.7% 7.3% 5.4% 7.3%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 27 27 27 0.82 3% Sample 2 200 207 196 193 199 5.24 2.6% Sample 3 441 428 410 450 432 15.03 3.4% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.016 0.077 0.147 0.253 0.571 0.840 1.824 2.587
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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