ELISA/assay

Human Angiogenin/ANG ELISA Kit PicoKine®

EK0305

96 wells/kit, with removable strips.

400美元

Human Angiogenin/ANG ELISA Kit PicoKine®

96 wells/kit, with removable strips.

400美元

Human Angiogenin/ANG ELISA Kit PicoKine® (96 Tests). Quantitate Human ANG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

人类

酶联免疫吸附测定

ANG

P03950

Expression system for standard: E.coli; Immunogen sequence: Q25-P147

There is no detectable cross-reactivity with other relevant proteins.

78 pg/ml - 5,000 pg/ml

<12 pg/ml

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

单克隆抗体来自小鼠, 多克隆抗体来自山羊

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Angiogenin

Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5;

Angiogenin

16550分子量

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.

Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.

Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.

Belongs to the pancreatic ribonuclease family.

Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

RNA Processing, Signal Transduction, Translation