产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human Angiogenin/ANG PicoKine ELISA Kit
目录 :
EK0305
规格 :
96 wells/kit, with removable strips.
价格 :
400美元
文章摘录数: 2
参考文献
Matter R, Abdelmaksoud A, Shams M, Bebawy E. Serum angiogenin level in sickle cell disease and beta thalassemia patients. Pediatr Hematol Oncol. 2014;31:50-6 pubmed 出版商
Yalcindag A, Gedik Oğuz Y, Yalçindağ F. The relationship between serum levels of angiogenin, bFGF, VEGF, and ocular involvement in patients with Behçet's disease. Graefes Arch Clin Exp Ophthalmol. 2013;251:1807-12 pubmed 出版商
产品信息
SKU号 :
EK0305
状态 :
Enabled
名称 :
Human Angiogenin/ANG PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
ANG
价格 :
400美元
试验范围 :
78pg/毫升5000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human Angiogenin/ANG with <12pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Angiogenin/ANG tissue specificity: Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ANG. 96wells/kit, with removable strips.
description1 :
Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human Angiogenin/ANG PicoKine Fast ELISA Kit and many others.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P03950
宿主 :
No
免疫原 :
Expression system for standard: E.coli; Immunogen sequence: Q25-P147
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<12pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
应用 :
酶联免疫吸附测定
反应物种 :
人类
图像标记 :
Human ANG PicoKine ELISA Kit standard curve
anticoagulant :
肝素或乙二胺四乙酸
assay procedure step 1 :
5000pg/毫升, 2500pg/毫升, 1250pg/毫升, 625pg/毫升, 312pg/毫升, 156pg/毫升, 78pg/毫升
背景 :
Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.
competitor equivalent skus :
sc 8357
研究种类 :
Rna Processing, Signal Transduction, Translation
同义词 :
Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5;
基因全称 :
Angiogenin
分子量 :
16550分子量
protein function :
Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
subcellular localization :
Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.
组织特异性 :
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
蛋白名 :
Angiogenin
sequence similarities :
Belongs to the pancreatic ribonuclease family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 107 719 2914 100 723 2957 Standard deviation 4.81 48.17 195.23 4.6 49.16 251.34 CV(%) 4.5% 6.7% 6.7% 4.6% 6.8% 8.5%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 107 121 121 109 114 6.53 5.7% Sample 2 719 697 632 755 700 44.76 6.3% Sample 3 2914 2847 2962 2827 2887 53.74 1.8% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0.0 78 156 313 625 1250 2500 5000 O.D. 0.047 0.116 0.169 0.265 0.506 0.927 1.534 2.375
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits