产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Rat BDNF PicoKine ELISA Kit
目录 :
EK0308
规格 :
96 wells/kit, with removable strips.
价格 :
270美元
文章摘录数: 1
参考文献
Etemad A, Sheikhzadeh F, Asl N. Evaluation of brain-derived neurotrophic factor in diabetic rats. Neurol Res. 2015;37:217-22 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0308 图像 1
Rat BDNF PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0308
状态 :
Enabled
名称 :
Rat BDNF PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
BDNF
价格 :
270美元
试验范围 :
31.2pg/毫升2000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Rat BDNF with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. BDNF tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat BDNF. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P23363
宿主 :
No
免疫原 :
Expression system for standard: sf21; Immunogen sequence: H129-R247
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<15pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate).
应用 :
酶联免疫吸附测定
反应物种 :
大鼠
前体活性 :
Bovine, Canine, Monkey, Rabbit
图像标记 :
Rat BDNF PicoKine ELISA Kit standard curve
anticoagulant :
肝素, 乙二胺四乙酸或柠檬酸盐
assay procedure step 1 :
4000pg/毫升, 2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升
背景 :
Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.
competitor equivalent skus :
sc 546 sc 33905 sc 33904 sc 20981
研究种类 :
Alzheimer'S Disease, Atherosclerosis, Cardiovascular, Diabetes, Diabetes Associated, Growth And Development, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Obesity
同义词 :
Brain-derived neurotrophic factor;BDNF;Bdnf;
基因全称 :
脑源性神经营养因子
分子量 :
28109分子量
protein function :
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS (By similarity).
subcellular localization :
Secreted.
蛋白名 :
脑源性神经营养因子
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 88 292 906 84 273 977 Standard deviation 4.4 16.06 70.66 4.2 19.11 77.18 CV(%) 5% 5.5% 7.8% 5% 7% 7.9%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 98 90 102 94 5.72 6% Sample 2 292 275 266 281 278 9.44 3.3% Sample 3 906 993 901 935 933 36.58 3.9% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.074 0.101 0.160 0.287 0.563 1.251 2.116
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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