产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human BMP-5 PicoKine ELISA Kit
目录 :
EK0310
规格 :
96 wells/kit, with removable strips.
价格 :
400美元
图像
图像 1 :
武汉博士德生物工程有限公司 EK0310 图像 1
Human BMP-5 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0310
状态 :
Enabled
名称 :
Human BMP-5 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
BMP5
价格 :
400美元
试验范围 :
156pg/毫升10000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human BMP5 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and bone tissue. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. BMP5 tissue specificity: Expressed in the lung and liver.
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human BMP-5. 96wells/kit, with removable strips.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
P22003
宿主 :
No
免疫原 :
Expression system for standard: NSO; Immunogen sequence: A317-H454
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<10pg/ml
kit components :
10ng/tube
样品类型 :
cell culture supernates, serum and bone tissue.
应用 :
酶联免疫吸附测定
反应物种 :
人类
前体活性 :
Bovine, Canine, Horse, Monkey, Rabbit
图像标记 :
Human BMP-5 PicoKine ELISA Kit standard curve
assay procedure step 1 :
10000pg/毫升, 5000pg/毫升, 2500pg/毫升, 1250pg/毫升, 625pg/毫升, 312pg/毫升, 156pg/毫升
背景 :
Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.
研究种类 :
Tgf Beta
同义词 :
骨形态发生蛋白5;BMP-5;BMP5;
基因全称 :
骨形态发生蛋白5
分子量 :
51737分子量
protein function :
Induces cartilage and bone formation.
subcellular localization :
Secreted.
组织特异性 :
Expressed in the lung and liver.
蛋白名 :
骨形态发生蛋白5
sequence similarities :
Belongs to the TGF-beta family.
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 242 1573 4363 266 1683 4290 Standard deviation 16.45 69.21 314.13 20.74 82.46 347.49 CV(%) 6.8% 4.4% 7.2% 7.8% 4.9% 8.1%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 228 246 239 238 6.68 2.8% Sample 2 1573 1794 1863 1590 1705 126.02 7.3% Sample 3 4363 4914 4157 4495 4482 276.84 6.1% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.003 0.101 0.143 0.275 0.437 0.889 1.521 2.526
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠多克隆抗体来自山羊
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits