产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
ELISA/assay
产品名称 :
Human BMP-2 PicoKine ELISA Kit
目录 :
EK0311
规格 :
96 wells/kit, with removable strips.
价格 :
400美元
文章摘录数: 1
参考文献
ToÅ¡ovský M, Bradna P, Andrýs C, Andrýsová K, Cermáková E, Soukup T. The VEGF and BMP-2 levels in patients with ankylosing spondylitis and the relationship to treatment with tumour necrosis factor alpha inhibitors. Acta Medica (Hradec Kralove). 2014;57:56-61 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 EK0311 图像 1
Human BMP-2 PicoKine ELISA Kit standard curve
产品信息
SKU号 :
EK0311
状态 :
Enabled
名称 :
Human BMP-2 PicoKine ELISA Kit
目录名称 :
Picokine ELISA Kits
基因名称 :
骨形态发生蛋白2
价格 :
400美元
试验范围 :
31.2pg/毫升2000pg/毫升
componentsneeded :
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
共轭标签 :
No
描述 :
Detect Human BMP2 with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: bone tissue, cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. BMP2 tissue specificity:
short description :
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human BMP-2. 96wells/kit, with removable strips.
description1 :
Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human BMP-2 PicoKine Fast ELISA Kit and many others.
规格 :
96 wells/kit, with removable strips.
UniProt数据库编号 :
C8C060
宿主 :
No
免疫原 :
Expression system for standard: CHO; Immunogen sequence: Q283-R396
储存 :
Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
交叉活性 :
There is no detectable cross-reactivity with other relevant proteins.
敏感性 :
<2pg/ml
kit components :
10ng/tube
样品类型 :
bone tissue, cell culture supernates and serum.
应用 :
酶联免疫吸附测定
反应物种 :
人类
前体活性 :
Bovine, Horse, Monkey, Rabbit
图像标记 :
Human BMP-2 PicoKine ELISA Kit standard curve
assay procedure step 1 :
2000pg/毫升, 1000pg/毫升, 500pg/毫升, 250pg/毫升, 125pg/毫升, 62.5pg/毫升, 31.2pg/毫升
背景 :
Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
研究种类 :
Angiogenesis, Cancer, Cardiovascular, Cytoskeleton / Ecm, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Neural Stem Cells, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, Tgf Beta
同义词 :
骨形态发生蛋白2;骨形态发生蛋白2;
基因全称 :
骨形态发生蛋白2
分子量 :
44702分子量
蛋白名 :
骨形态发生蛋白2
intra inter assay cv :
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 55 250 1005 59 255 1061 Standard deviation 4.29 13.25 70.35 4.66 15.55 83.81 CV(%) 7.8% 5.3% 7% 7.9% 6.1% 7.9%
再现性 :
Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 51 58 51 53 2.94 5.5% Sample 2 250 245 240 275 252 13.46 5.3% Sample 3 1005 885 885 952 931 50.36 5.4% *number of samples for each test n=16.
样品数据 :
Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.026 0.060 0.114 0.349 0.808 1.452 2.489
tmb incubation time :
15-25min
principle :
单克隆抗体来自小鼠单克隆抗体来自小鼠
更新 :
3/7/19 6:53
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits